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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/10780

Title: Isolation, Identification and Characterization of Some Petroleum Metabolizers from Soils Contaminated with Petroluem Oils in Kumasi Metropolis- Ghana
Authors: Asiedu, Nana Yaw
Asiedu, Yaa Adoma
Bediako, Phanuel Coffie Kofi
Keywords: Isolation
Waste Petroleum Oils
Issue Date: 2014
Publisher: Journal of Microbiology Research
Citation: Journal of Microbiology Research 2014, 4(2): 117-124 DOI: 10.5923/j.microbiology.20140402.12
Abstract: Micro-organisms which are believed to metabolize waste petroleum oils from soaked soils in some areas in Kumasi metropolis-Ghana were studied. The studies investigate the isolation, characterization and mode of action of the organisms with petroleum contaminated soils using available equipment and information to their appropriate genera. The analysis classified the isolates into the aerobic and micro-aerophilic categories. Isolates metabolic activities on the various petroleum fractions (petrol, kerosene, engine oil, and diesel) gave the following percentages for profuse growth: 0.1-10%-petrel, 0.1-3.8% -kerosene, 0.1-2.3%-engine oil and 0.1-3.8% -diesel. Morphological characteristics of the isolates were identical to the average dimension of colonies ranging between 3.00mm and 4.90mm and cell sizes between 0.70mm and 1.60mm. The isolates showed gram-negative reactions. When the isolates were cultured for the same period and using the equal amounts of suspension, petrol gave 4.21 x 105 cells/ml suspension, engine oil gave 3.78 x 105 cells/ml suspension, kerosene gave 2.64 x 105 cells/ml suspension and diesel oil gave 1.50 x105 cells /ml suspension by total microbial count techniques. The isolates thus studied appear to belong to the following families: Pseudomonadaceae, Asotobacteraraceae, Methylomodaceae and Halobacteraceae.
Description: An article published by Journal of Microbiology Research 2014, 4(2): 117-124 DOI: 10.5923/j.microbiology.20140402.12
URI: http://hdl.handle.net/123456789/10780
Appears in Collections:College of Engineering

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