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|Title: ||Anti-proliferative effect of isoflavones isolated from soybean and soymilk powder on lymphoma (DG 75) and leukemia (CEM) cell lines|
|Authors: ||C. Apprey1, R. A. Annan1 and|
Arthur, F. K. N.
|Issue Date: ||2015|
|Publisher: ||British Journal of Pharmaceutical Research|
|Citation: ||British Journal of Pharmaceutical Research 7(3): 206-216, 2015, Article no.BJPR.2015.103|
|Abstract: ||Aim: Isoflavones in soybean have been linked to reduced risk of some cardiovascular diseases as well as some type of cancers. Extensive epidemiological, in-vitro and animal data suggests lower incidence of cardiovascular disease, hormone-dependent cancers of the breast and prostate, for consumers of soy and soy products. In this study, isoflavones were isolated from Soybean (SB) and soymilk powder (SMP) and the anti-proliferative effects of these isoflavones on Burkitt’s lymphoma (DG-75) and Leukemia (CEM) cell lines were investigated.
Place of Study: Department of Clinical Pathology, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra, Ghana between June and December, 2014.
Methodology: Isoflavanoids were isolated and HPLC done to confirm the isolate. Isoflavanoids were isolated form SB and SMP and the anti-proliferative effect of isoflavanoid isolated from SB, SMP, pure genistein and daidzein on lymphoma and leukaemia cells were compared using tetrazolium-based colorimetric (MTT) assay.
Results: The inhibitory effect of genistein on leukemia (CEM) cell line was stronger (IC50 = 28.4 µM) than daidzein (IC50 = >100 µM). For the DG 75, genistein had a better effect (IC50 = 11.05 µM than daidzein (IC50> 100 µM). The IC50 for SMP were 193.92 µg/ml (DG-75) and 54.17 µg/ml (CEM). In terms of selectivity index (SI), genistein had the highest (>6.95) on CEM followed by genistein (>3.52) and then SB (>1.16).
Conclusion: SMP had a better anti-proliferative effect on both CEM and DG-75 compared with SB.|
|Description: ||This article was published in the British Journal of Pharmaceutical Research 7(3): 206-216, 2015, Article no.BJPR.2015.103|
|Appears in Collections:||College of Science|
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