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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11040

Title: Medicinal plants used in wound care: Assessment of wound healing and antimicrobial properties of Zanthoxylum leprieurii
Authors: Agyare, Christian
Kisseih, Emelia
Kyere, Ivy Yaa
Ossei, Paul Poku Samapane
Keywords: Antimicrobial
Antioxidant
Wound healing
Minimum inhibitory concentration
Issue Date: Sep-2014
Publisher: Issues in Biological Sciences and Pharmaceutical Research
Citation: Issues in Biological Sciences and Pharmaceutical Research Vol.2(8),pp.081-089,September 2014; Available online at http://www.journalissues.org/IBSPR/ http://dx.doi.org/10.15739/ibspr.002
Abstract: Zanthoxylum leprieurii Guill and Perr. (Family Rutaceae) is used in traditional medicine as a diuretic, purgative and in the treatment of wounds, ulcers, pains, arthritis, skin and urinary tract infections, dysentery and intestinal worm infestation. However, no extensive scientific research has been conducted to verify the anti-infective and wound healing properties of the plant. The objective of this study is to investigate the antimicrobial, antioxidant and in vivo wound healing properties of the aqueous-methanol (3:7, v/v) stem bark of extract of Zanthoxylum leprieurii (AMZL). Minimum inhibitory concentration (MIC) of aqueous-methanol stem bark extract of Z. leprieurii against typed strains of Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and clinical strains of Streptococcus pyogenes and Candida albicans were 20.0, 20.0, 20.0, 40.0, 20.0 and 20.0 mg/mL respectively. The free radical scavenging activity using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) gave an IC50 of 28.7 μg/mL for the aqueous-methanol stem bark extract of Z. leprieurii while the reference antioxidant, α-tocopherol, had IC50 of 0.79 μg/ml. The excision wound model studies of the 7.5 and 15.0% w/w extract (AMZL) creams with Sprague-Dawley rats showed a significant decrease in wounds treated with 7.5% w/w aqueous cream extract at days 9 (p<0.001) and 11 (p<0.01) compared to the untreated wounds. There was profuse proliferation of fibroblasts with varying degrees of fibrosis in extract treated wound tissues compared with the untreated wound tissues. Fibroblasts and collagen fibers were more present in the extract treated groups compared to untreated wound tissues. Tissues from wounds treated with 7.5% w/w extract cream showed more fibrosis (60 to 70%) than the 15% w/w extract cream (40 to 50%). Preliminary phytochemical screening of the stem bark revealed the presence of alkaloids, flavonoids, carbohydrates and saponins. The above findings may justify the folkloric uses of the plant for the treatment of wounds and microbial infections.
Description: An article published by Issues in Biological Sciences and Pharmaceutical Research Vol.2(8),pp.081-089,September 2014 Available online at http://www.journalissues.org/IBSPR/ http://dx.doi.org/10.15739/ibspr.002
URI: http://hdl.handle.net/123456789/11040
Appears in Collections:College of Health Sciences

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