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|Title: ||Caspase inhibitors affect the kinetics and dimensions of tracheary elements in xylogenic Zinnia (Zinnia elegans) cell cultures|
|Authors: ||Twumasi, Peter|
Lakimova, Elena T.
Leperen, Wim van
Schel, Jan H.N.
Emons, Anne Mie C.
Kooten, Olaf van
Woltering, Ernst J.
|Issue Date: ||2010|
|Publisher: ||BMC Plant Biology|
|Citation: ||BMC Plant Biology 2010, 10:162 http://www.biomedcentral.com/1471-2229/10/162|
|Abstract: ||Background: The xylem vascular system is composed of fused dead, hollow cells called tracheary elements (TEs)
that originate through trans-differentiation of root and shoot cambium cells. TEs undergo autolysis as they
differentiate and mature. The final stage of the formation of TEs in plants is the death of the involved cells, a
process showing some similarities to programmed cell death (PCD) in animal systems. Plant proteases with
functional similarity to proteases involved in mammalian apoptotic cell death (caspases) are suggested as an
integral part of the core mechanism of most PCD responses in plants, but participation of plant caspase-like
proteases in TE PCD has not yet been documented.
Results: Confocal microscopic images revealed the consecutive stages of TE formation in Zinnia cells during transdifferentiation.
Application of the caspase inhibitors Z-Asp-CH2-DCB, Ac-YVAD-CMK and Ac-DEVD-CHO affected the
kinetics of formation and the dimensions of the TEs resulting in a significant delay of TE formation, production of
larger TEs and in elimination of the ‘two-wave’ pattern of TE production. DNA breakdown and appearance of
TUNEL-positive nuclei was observed in xylogenic cultures and this was suppressed in the presence of caspase
Conclusions: To the best of our knowledge this is the first report showing that caspase inhibitors can modulate
the process of trans-differentiation in Zinnia xylogenic cell cultures. As caspase inhibitors are closely associated with
cell death inhibition in a variety of plant systems, this suggests that the altered TE formation results from
suppression of PCD. The findings presented here are a first step towards the use of appropriate PCD signalling
modulators or related molecular genetic strategies to improve the hydraulic properties of xylem vessels in favour
of the quality and shelf life of plants or plant parts.|
|Description: ||An article published by BMC Plant Biology 2010, 10:162;
Available online at: http://www.biomedcentral.com/1471-2229/10/162|
|Appears in Collections:||College of Science|
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