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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11056

Title: Assessing microbiological contamination of drinking water from Barekese Water Treatment Plant in the Kumasi Metropolis using PCR and microbial culturing techniques
Authors: Twumasi, Peter
Prasad, R.
Nyako, M. O.
Amoako, T. N. E.
Talukdar, S.
Keywords: Polymerase chain reaction (PCR)
Thermal cycler
viable but nonculturable (VBNC)
Escherichia coli
Shiga toxin, bacterial culture
Water treatment
Issue Date: 2014
Publisher: Ghana Journal of Chemistry
Citation: Ghana Journal of Chemistry, Vol. 2, No. 1 (2014)
Abstract: The intake of water contaminated by pathogenic microbes has led to a variety of severe waterborne ailments in Ghana and the African continent at large. Despite the short comings associated with the sole dependence of culture analysis of pathogens in water treatment plants, it remains the number one technique used in almost all treatment plants in the nation and beyond for microbial screening and analysis. Water samples collected at Barekese Water Treatment Plant in Kumasi, Ghana, were analysed for the presence of Shiga Toxin producing Escherichia coli (STEC) using both bacterial cell culture and PCR amplification techniques. Four out of nine samples (44.44%) were positive for STEC by PCR whiles three samples (33.33%) were positive for STEC by the culture technique. The results obtained have shown PCR to be an effective tool in detecting microbial contamination in water samples as compared to the classical culturing methods currently in use in all water treatment plants in Ghana.
Description: An article published by Ghana Journal of Chemistry, Vol. 2, No. 1 (2014)
URI: http://hdl.handle.net/123456789/11056
Appears in Collections:College of Science

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