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|Title: ||Prevalence of papC and usp Virulence Factors in Uropathogenic Escherichia coli Causing Asymptomatic Urinary Tract Infections in Adolescents|
|Authors: ||Darko, Samuel Nkansah|
|Issue Date: ||2013|
|Publisher: ||British Microbiology Research Journal|
|Citation: ||British Microbiology Research Journal 3(3): 423-430, 2013|
|Abstract: ||Aims: To determine the prevalence of two virulence genes associated with uropathogenic
Escherichia coli; papC gene of the P fimbriae for adherence to uro-epithelial cells and usp
(uropathogen-specific protein) gene, a Vibrio cholerae toxin gene homologue.
Study Design: Cross sectional.
Place and Duration of Study: Department of Biochemistry and Biotechnology and the
Clinical Analysis Laboratory, Kwame Nkrumah University of Science and Technology,
Kumasi, between October 2011 and February 2012.
Methodology: Escherichia coli isolates (n= 149) from an adolescent population of ages 13-
18 years (from a total sampled population of 85 males and 64 females) were screened for
papC and usp, using specific primers for the two genes in polymerase chain reactions.
Results: The usp gene was the most prevalent (72.48%), followed by papC (51.00%) and
papC+usp (24.16%). Significant difference (P = .002) was observed between papC and usp
and also papC and papC+usp (P < .0001). usp Gene prevalence was also significantly
different from that of papC+usp (P < .0001).Conclusion: This study suggests that a higher proportion of strains of uropathogenic
Escherichia coli implicated in UTI in the studied population possess the usp gene whose
protein product potentially serves to reduce competing microbes in the urinary tract.|
|Description: ||An article published by British Microbiology Research Journal
3(3): 423-430, 2013|
|Appears in Collections:||College of Science|
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