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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11119

Title: Anti-proliferative Effect of Isoflavones Isolated from Soybean and Soymilk Powder on Lymphoma (DG 75) and Leukemia (CEM) Cell Lines
Authors: Apprey, Charles
Larbie, C.
Arthur, F. K. N.
Appiah-Opong, R.
Annan, R. A.
Tuffour, I.
Keywords: Soybean
Issue Date: 2015
Publisher: British Journal of Pharmaceutical Research
Citation: British Journal of Pharmaceutical Research 7(3): 206-216, 2015, Article no.BJPR.2015.103
Abstract: well as some type of cancers. Extensive epidemiological, in-vitro and animal data suggests lower incidence of cardiovascular disease, hormone-dependent cancers of the breast and prostate, for consumers of soy and soy products. In this study, isoflavones were isolated from Soybean (SB) and soymilk powder (SMP) and the anti-proliferative effects of these isoflavones on Burkitt’s lymphoma (DG-75) and Leukemia (CEM) cell lines were investigated. Place of Study: Department of Clinical Pathology, Noguchi Memorial Institute of Medical Research, University of Ghana, Accra, Ghana between June and December, 2014. Methodology: Isoflavanoids were isolated and HPLC done to confirm the isolate. Isoflavanoids were isolated form SB and SMP and the anti-proliferative effect of isoflavanoid isolated from SB, SMP, pure genistein and daidzein on lymphoma and leukaemia cells were compared using tetrazolium-based colorimetric (MTT) assay. Results: The inhibitory effect of genistein on leukemia (CEM) cell line was stronger (IC50 = 28.4 μM) than daidzein (IC50 = >100 μM). For the DG 75, genistein had a better effect (IC50 = 11.05 μM than daidzein (IC50> 100 μM). The IC50 for SMP were 193.92 μg/ml (DG-75) and 54.17 μg/ml (CEM). In terms of selectivity index (SI), genistein had the highest (>6.95) on CEM followed by genistein (>3.52) and then SB (>1.16). Conclusion: SMP had a better anti-proliferative effect on both CEM and DG-75 compared with SB.
Description: An article published by British Journal of Pharmaceutical Research 7(3): 206-216, 2015, Article no.BJPR.2015.103
URI: http://hdl.handle.net/123456789/11119
ISSN: 2231-2919
Appears in Collections:College of Science

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