KNUSTSpace >
Research Articles >
College of Science >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11140

Title: Pectin isolation and characterization from six okra genotypes
Authors: Kpodo, F. M.
Agbenorhevi, Jacob K.
Alba, K.
Bingham, R.J.
Oduro, I. N.
Morris, G.A.
Keywords: Okra
Issue Date: 2017
Publisher: Food Hydrocolloids
Citation: Food Hydrocolloids, 72 (2017) ,323e330
Abstract: Pectin was isolated by aqueous extraction at pH 6.0 from the pods of six different okra genotypes (Abelmoschus esculentus L.). Genetic diversity was determined using fragment length analysis (FLA) of ten simple sequence repeat (SSR) markers. Physical and chemical evaluation of pectin was performed by means of FT-IR and NMR spectroscopy, sugar composition analysis (GC-MS), size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALLS), dilute solution viscometry and steady shear rheology assisted by principal component analysis (PCA). Each of the SSR markers detected on average 4.1 alleles and revealed unique genotypes for each sample. Extraction yield was between 11 and 14% resulting in pectin with galacturonic acid content between 43 and 63%, low degree of methylesterification (17e25%) and high degree of acetylation (20e40%). All samples were of high weightaverage molar mass (Mw) (700e1700 103 g mol 1) and sugar composition analysis revealed the structural diversity of samples with HG/RG-I ratios ranging between 1.3 and 3.1. The present work shows that individual okra genotypes provide pectin with different structural properties that could potentially provide a new source of functional pectin for the food or pharmaceutical industries.
Description: An article published by Food Hydrocolloids, 72;(2017), 323e330
URI: http://hdl.handle.net/123456789/11140
Appears in Collections:College of Science

Files in This Item:

File Description SizeFormat
Pectin_isolation_and_characterization_from_six_okr.pdf867.14 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback