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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11154

Title: Isolation and characterization of soilborne virulent bacteriophages infecting the pathogen Rhodococcus equi
Authors: Salifu, Samson Pandam
Casey, S. A. Campbell
Foley, S.
Issue Date: 2013
Publisher: Journal of Applied Microbiology
Citation: Journal of Applied Microbiology, 114,1625--1633, doi:10.1111/jam.12194
Abstract: Aims: To isolate and characterize a diversity of bacteriophages (phages) that infect the soilborne pathogen Rhodococcus equi. Methods and Results: Twenty-seven phages were isolated from soil samples from geographically distinct locations using a range of R. equi bacterial strains, including clinical isolates. On the basis of host range, genomic DNA restriction profiles and virion protein profiles, the diversity of these phages was extensive, with phages being divided into 16 groupings. Conclusions: Based on a range of criteria, these phages could be divided into 16 distinct groupings. The majority of the phages recovered from soil were Siphoviridae, adding to the limited number of Siphoviridae described to date for R. equi. One grouping consisted of phages belonging to the Myoviridae. Significance and Impact of the Study: This represents the first study looking at the diversity of phages infecting the pathogen R. equi, including the first Myoviridae to be isolated and characterized for the genus Rhodococcus and for the nonmycobacterial actinomycetes. Given their diverse host range, including clinical isolates, this collection of phages offers the potential for the development of phage cocktails for use as a therapeutic or alternatively in the biocontrol of this pathogen in reservoirs of infection relating to animal husbandry.
Description: An article published by Journal of Applied Microbiology, 114,1625-1633, doi:10.1111/jam.12194
URI: http://hdl.handle.net/123456789/11154
ISSN: 1364-5072
Appears in Collections:College of Science

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