Research Articles >
College of Health Sciences >
Please use this identifier to cite or link to this item:
|Title: ||In vitro and in vivo antioxidant properties of Phyllanthus muellerianus and its major constituent, geraniin|
|Authors: ||Boakye, Yaw Duah|
Dapaah, Susana Oteng
|Issue Date: ||2016|
|Publisher: ||Oxidants and Antioxidants in Medical Science|
|Citation: ||Oxidants and Antioxidants in Medical Science, 5(2): 70-78; DOI: 10.5455/oams.290616.or.099|
|Abstract: ||Objective: Phyllanthus muellerianus (Kuntze) Exell. of the family Euphorbiaceae, is used as a wound healing
agent in Ghana and other West African countries. Oxidative stress has been identified to delay wound healing
process and subsequently leads to the development of chronic wounds. Hence, the aim of the present study was
to investigate the in vivo and in vitro antioxidant properties of aqueous extract of the aerial parts of P.muellerianus
(PLE) and its major constituent, geraniin.
Methods: Wound tissues excised from rats treated with PLE (0.25, 0.5 and 1% w/w) and geraniin (0.1, 0.2 and
0.4 % w/w) incorporated in aqueous creams were assayed for the levels of superoxide dismutase (SOD), catalase
(CAT), myeloperoxidase (MPO), ascorbate peroxidase (APx) and malondialdehyde (MDA). 1,1-diphenyl-2-picrylhydrazyl
(DPPH), ferric reducing antioxidant power, total phenolic content and total antioxidant capacity assays
were used to measure in vitro antioxidant activity.
Results: SOD, CAT and APx activity increased significantly in both PLE and geraniin-treated wound tissues
compared to the untreated tissues. However, MPO activity decreased significantly in all three PLE and 0.2 and
0.4% w/w geraniin-treated wound tissues compared to the untreated tissues. MDA levels decreased significantly
in all of the PLE and geraniin-treated wound tissues compared to the untreated tissues. Ferric reducing antioxidant
power (FRAP) was observed to be significantly lower for PLE than geraniin.
Conclusion: PLE and geraniin exhibit both in vivo and in vitro antioxidant properties.|
|Description: ||An article published by Oxidants and Antioxidants in Medical Science, 5(2): 70-78; DOI: 10.5455/oams.290616.or.099|
|Appears in Collections:||College of Health Sciences|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.