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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11244

Title: Ellagitannins from Phyllanthus muellerianus (Kuntze) Exell.: Geraniin and furosin stimulate cellular activity, differentiation and collagen synthesis of human skin keratinocytes and dermal fibroblasts
Authors: Agyare, Christian
Lechtenberg, Matthias
Deters, Alexandra
Petereit, Frank
Hensel, Andreas
Keywords: Phyllanthus muellerianus (Kuntze) Exell.
Euphorbiaceae
Keratinocytes
Skin fibroblasts
Geraniin
Furosin
Collagen
Differentiation
Proliferation
Issue Date: 2011
Publisher: Phytomedicine
Citation: Phytomedicine, 18 (2011); 617–624
Abstract: Leaves from Phyllanthus muellerianus (Kuntze) Exell. are traditionally used for wound healing in Western Africa. Aqueous extracts of dried leaves recently have been shown to stimulate proliferation of human keratinocytes and dermal fibroblasts. Within bioassay-guided fractionation the ellagitannins geraniin (1), corilagin (2), furosin (3), the flavonoids quercetin-3-O- -d-glucoside (isoquercitrin), kaempferol-3- O- -d-glucoside (astragalin), quercetin-3-O-d-rutinoside (rutin), gallic acid, methyl gallate, caffeic acid, chlorogenic acid, 3,5-dicaffeoylquinic acid and caffeoylmalic acid (phaselic acid) have been identified in P. muellerianus for the first time. Geraniin was shown to be the dominant component of an aqueous extract. Suitable analytical methods for quality control of geraniin in P. muellerianus extract (methanol/water, 70/30) have been developed and validated based on ICH guidelines (ICH-compliant protocol). Geraniin and furosin increased the cellular energy status of human skin cells (dermal fibroblasts NHDF, HaCaT keratinocytes), triggering the cells towards higher proliferation rates, with fibroblasts being more sensitive than keratinocytes. Highest stimulation of NHDF by geraniin was found at 5 M, and of keratinocytes at 50–100 M. Furosin stimulated NHDF at about 50 M, keratinocytes at about 150–200 M. Necrotic cytotoxicity of geraniin, as measured by LDH release, was observed at 20 M for NHDF and 150 M for keratinocytes. Toxicity of furosin – less than that of geraniin – was observed at >400 M. Furosin and geraniin stimulated the biosynthesis of collagen from NHDF at 50 Mand 5–10 Mrespectively. Geraniin at 105 Msignificantly stimulated the differentiation in NHEK while furosin had a minor influence on the expression of involucrin and cytokeratins K1 and K10. The study proves clearly that hydrophilic extracts from P. muellerianus and especially the lead compound geraniin exhibit stimulating activity on dermal fibroblasts and keratinocytes, leading to increased cell proliferation, barrier formation and formation of extracellular matrix proteins. From these findings the traditional clinical use of such extracts for wound healing seems to be justified.
Description: An article published by Phytomedicine, 18 (2011); 617–624
URI: http://hdl.handle.net/123456789/11244
Appears in Collections:College of Health Sciences

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