DSpace
 

KNUSTSpace >
Research Articles >
College of Health Sciences >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11247

Title: An ethnopharmacological survey and in vitro confirmation of ethnopharmacological use of medicinal plants used for wound healing in Bosomtwi-Atwima-Kwanwoma area, Ghana
Authors: Agyare, Christian
Asase, Alex
Lechtenbergc, Matthias
Niehues, Michael
Deters, Alexandra
Hensel, Andreas
Keywords: Wound-healing
Ghana
Phyllanthus muellerianus
Combretum smeathmanii
Pycnanthus angolensis
Fibroblasts
Keratinocytes
Helicobacter pylori
Bacterial adhesion
Issue Date: 2009
Publisher: Journal of Ethnopharmacology
Citation: Journal of Ethnopharmacology, 125 (2009), 393–403
Abstract: Aims of the study: Wounds represent a major health burden and drain on healthcare resources in the world including Ghana and Africa. The majority of the people of Ghana and Africa still patronize traditional medicine for their health needs including various forms of wounds. The aim of this study is the identification ofmedicinal plants, type ofwounds, dosage forms and collectionmethods used traditionally in treating wounds in the Bosomtwi-Atwima-Kwanwoma district, Ghana. In vitro screening of selected extracts from these plants on cell physiology of human dermal fibroblasts and keratinocytes was to be performed. Materials and methods: Validated questionnaires were administered to 78 traditional healers in 54 communities of the district. Interviews and structured conversations were used to administer the questionnaires. Selected herbal material dominantly used by the healerswas collected, identified and aqueous and ethanolic extractswere investigated in vitro on influence on cell physiology of keratinocytes and dermal fibroblasts (MTT-, BrdU-, LDH-assay). Antioxidant activities of ethanolic extractswere determined by free radical scavenging activity. Antiadhesive activity against Helicobacter pylori on human stomach cells was investigated for extracts reported to be used for stomach ulcer treatment. Results: The ethnopharmacological survey revealed 104 plants species belonging to 47 families. The detailed use of these plants is documented. Aqueous extracts of Phyllanthus muellerianus, Pycnanthus angolensis and Combretum smeathmanni influenced the mitochondrial activity and proliferation of dermal fibroblasts and keratinocytes significantly. Ethanolic extracts of selected plants exhibited strong antioxidant activities comparable to -tocopherol. For Spathodea campanulata, Hoslundia opposita and Pycnanthus angolensis, which were reported by the healers to be used also for wound healing in case of stomach ulcers, strong antiadhesive activity against Helicobacter pylori was demonstrated, while the extracts did not exhibit any direct cytotoxicity against the bacterium. Conclusions: Traditional use of many wound-healing plants from Ghana can be well rationalized by the in vitro investigation of aqueous extracts. E.g. extracts of Phyllanthus muellerianus, Pycnanthus angolensis and Combretum smeathmanni exhibited significant influence on the cell viability and proliferation of keratinocytes and dermal fibroblasts.
Description: An article published by Journal of Ethnopharmacology, 125 (2009), 393–403
URI: http://hdl.handle.net/123456789/11247
Appears in Collections:College of Health Sciences

Files in This Item:

File Description SizeFormat
biblio-ha-40-agyare.pdf617.93 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback