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|Title: ||Antimicrobial and Resistance Modifying Activities of Cerevisterol Isolated from Trametes Species|
|Authors: ||Appiah, Theresa|
Boamah, Vivian Etsiapa
Boakye, Yaw Duah
|Issue Date: ||2018|
|Publisher: ||Bentham Science Publishers|
|Citation: ||Current Bioactive Compounds 2018, 14|
|Series/Report no.: ||14;|
|Abstract: ||Abstract: Background: The development of resistance by pathogenic microorganisms has renewed the
worldwide search for novel antimicrobial agents. Mushrooms are of recent interest because a wide variety
of biologically active compounds have been isolated from them. This study isolated antimicrobial
compound from two wood decaying mushrooms, Trametes gibbosa and Trametes elegans, and determined
the resistance modifying activities of the isolated compound.
Methods: Bioactivity guided isolation of active principles from the methanol extract of T. gibbosa and
T. elegans was performed using column and preparative high-performance liquid chromatography. The
structures of isolated compounds were elucidated using nuclear magnetic resonance spectroscopy. Broth
micro-dilution assay was used to determine the antimicrobial and resistance modifying activities of the
isolated compounds against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella
typhi, Streptococcus pyogenes, Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Candida
albicans, Aspergillus niger, Aspergillus flavus and Aspergillus tamarii.
Results: Bioactivity guided isolation lead to the isolation of cerevisterol (ergosta-7, 22E-diene-35, 6-
triol) from both T. gibbosa and T. elegans. The isolated cerevisterol inhibited the growth of S. typhi, S.
aureus and A. niger with MICs of 25 g/mL each and 50 g/mL against E. faecalis. The MBCs of
cerevisterol against S. typhi S. aureus, E. faecalis and A. niger were 50, 100, 200 and 100 g/mL, respectively.
The sub-inhibitory concentration (3 g/mL) of cerevisterol modified the activity of erythromycin,
ampicillin, ciprofloxacin, tetracycline and amoxicillin either by potentiating or reducing their activities.|
|Description: ||Article published in Current Bioactive Compounds 2018, 14. Available at 10.2174/1573407214666180813101146|
|Appears in Collections:||College of Health Sciences|
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