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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11811

Title: HT-SPOTi: A rapid drug susceptibility unit 17.8 test (dst) to evaluate antibiotic resistance profiles and novel chemicals for anti-infective drug discovery
Keywords: drug susceptibility testing (DST)
antimicrobial resistance
whole-cell phenotypic evaluation
infectious diseases
drug discovery
Issue Date: 12-Feb-2016
Publisher: Current Protocols in Microbiology
Citation: Curr. Protoc. Microbiol. 40:17.8.1-17.8.12.
Abstract: Antibiotic resistance is one of the major threats to global health and wellbeing. The past decade has seen an alarming rise in the evolution and spread of drug-resistant strains of pathogenic microbes. The emergence of extensively drug resistant (XDR) strains of Mycobacterium tuberculosis and antimicrobial resistance among the ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter species) as well as fungal pathogens (such as certain species of Candida, Aspergillus, Cryptococcus, and Trichophyton) poses a significant 21st century scientific challenge. With an extremely limited arsenal of efficacious antibiotics, techniques that can (a) identify novel antimicrobials and (b) detect antimicrobial resistance are becoming increasingly important. In this article, we illustrate the HT-SPOTi, an assay that is principally based on the growth of an organism on agar medium containing a range of different concentrations of drugs or inhibitors. The simple methodology makes this assay ideal for evaluating novel antimicrobial compounds as well as profiling an organism’s antibiotic resistance profile.
Description: An article published by Curr. Protoc. Microbiol. 40:17.8.1-17.8.12.and available at doi: 10.1002/9780471729259.mc1708s40
URI: http://hdl.handle.net/123456789/11811
Appears in Collections:College of Health Sciences

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