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|Title: ||Detection of Mycolactone A/B in Mycobacterium ulcerans–Infected Human Tissue|
|Authors: ||Sarfo, Fred Stephen|
Phillips, Richard O.
Mahrous, Engy A.
Lee, Richard E.
|Issue Date: ||5-Jan-2010|
|Publisher: ||PLoS Neglected Tropical Diseases|
|Citation: ||PLoS Negl Trop Dis 4(1): e577. doi:10.1371/journal.pntd.0000577|
|Abstract: ||Background: Mycobacterium ulcerans disease (Buruli ulcer) is a neglected tropical disease common amongst children in
rural West Africa. Animal experiments have shown that tissue destruction is caused by a toxin called mycolactone.
Methodology/Principal Findings: A molecule was identified among acetone-soluble lipid extracts from M. ulcerans (Mu)-
infected human lesions with chemical and biological properties of mycolactone A/B. On thin layer chromatography this
molecule had a retention factor value of 0.23, MS analyses showed it had an m/z of 765.6 [M+Na+] and on MS:MS
fragmented to produce the core lactone ring with m/z of 429.4 and the polyketide side chain of mycolactone A/B with m/z
of 359.2. Acetone-soluble lipids from lesions demonstrated significant cytotoxic, pro-apoptotic and anti-inflammatory
activities on cultured fibroblast and macrophage cell lines. Mycolactone A/B was detected in all of 10 tissue samples from
patients with ulcerative and pre-ulcerative Mu disease.
Conclusions/Significance: Mycolactone can be detected in human tissue infected with Mu. This could have important
implications for successful management of Mu infection by antibiotic treatment but further studies are needed to measure
|Description: ||An article by PLoS
Neglected Tropical Diseases|
|Appears in Collections:||College of Health Sciences|
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