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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12068

Title: Evaluation of anti-inflammatory and antioxidant activity of Furanocoumarins and Sterolin from the stem bark of Ficus exasperata Vahl (Moraceae)
Authors: Amponsah, Isaac K
Fleischer, Theophilus C
Dickson, Rita A.
Annan, Kofi
Thoss, Vera
Keywords: Anti-inflammatory
Antioxidan
DPPH
Bergapten
F. exasperata
Oxypeucedanin hydrate
Issue Date: Oct-2013
Publisher: Journal of Scientific and Innovative Research
Citation: Journal of Scientific and Innovative Research, 2013; 2 (5): 880-887
Abstract: Ficus exasperata is a popular plant among traditional healers in Africa where various parts of the plant are used for some medicinal purposes. Previous research on the stem bark revealed considerable anti-inflammatory, antioxidant and antimicrobial activities, which resided in the chloroform extract of the plant. Although, the plant enjoys a battery of pharmacological activities, it has rarely been studied for it bioactive secondary metabolites. The study was designed to fractionate the bioactive chloroform extract of the stem bark, isolate and characterize some compounds which may be responsible for these activities. Fractionation and isolation of the compounds was done using gravity column and thin layer chromatographic techniques on silica gel. The structures of the isolated constituents were elucidated using UV, mass spectrometry, 1D and 2D-NMR techniques. The carrageenan-induced foot oedema test in 7-day old chicks was used to assess the anti-inflammatory effect of the compounds. The in vitro antioxidant properties were evaluated using the DPPH radical scavenging assay. Bergapten, oxypeucedanin hydrate and the sterolin sitosterol-3-O-β-D-glucopyranoside were isolated as the active metabolites. They exhibited dose-dependent anti-inflammatory activities with respective ED50 values of 101.6±0.003, 126.4±0.011 and 225.9±0.012 mg/kg. They also showed concentration-dependent DPPH scavenging effect with IC50 of 63.38 ± 0.010 and 46.63 ± 0.011 for bergapten and oxypeucedanin hydrate respectively. Sitosterol 3-O-glucopyranoside showed the lowest activity. Bergapten, oxypeucedanin hydrate and the sterolin sitosterol-3-O-β-Dglucopyranoside display anti-inflammatory and antioxidant activities and thus contribute substantially to the bioactivities of the stem bark of F. exasperata
Description: This article is published in Journal of Scientific and Innovative Research
URI: http://hdl.handle.net/123456789/12068
ISSN: 2320-4818
Appears in Collections:College of Health Sciences

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