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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12306

Title: Assessment of platelet indices and platelet activation markers in children with Plasmodium falciparum malaria
Authors: Asare, Renate
Opoku‑Okrah, Clement
Owusu Danquah, Kwabena
Opare‑Sem, Ohene
Addai‑Mensah, Otchere
et. al
Keywords: Platelet membrane glycoproteins,
Platelet indices,
Plasmodium falciparum,
Flow cytometry
Issue Date: Dec-2020
Publisher: BioMed Central
Citation: Asare et al. Malar J (2020) 19:143 https://doi.org/10.1186/s12936-020-03218-4
Abstract: Background: Plasmodium falciparum malaria remains one of the world’s major infectious diseases that cause most morbidity and mortality, particularly in children. In Ghana, most children below the ages of 5 years depending on the severity of the infection often lose their lives. However, it is still debatable why infection with falciparum malaria contributes to thrombocytopenia. Methods: This study sought to investigate the expression of the various platelet indices and activation markers in children with falciparum malaria. Platelet indices (Platelet count [PLT], Plateletcrite [PCT], Mean Platelet Volume [MPV], Platelet Distribution Width [PDW] and Platelet-Large Cell Ratio [P-LCR]) and platelet surface membrane glycoproteins (GPIIb/IIIa [PAC-1], P-selectin [CD62p] and GPIV [CD36]) expressions were determined in children with falciparum malaria (cases) and healthy children (controls) using automated blood cell analysis and flow cytometry techniques, respectively. Results: Except for P-LCR, all the other platelet indices (PLT, MPV, PDW, and PCT) were significantly lower in the cases than the controls (P < 0.05). Also, it was observed that the level of expression of the activation markers; PAC 1 and CD62p showed a significant (P < 0.05) decreased before and after activation in falciparum malaria cases than in the controls. On the contrary, CD36 expression in the controls did not differ significantly (p > 0.05) from the malaria cases. Platelet activation markers were known to be associated with increased risk of falciparum malaria with the mean fluorescence intensity of PAC1 (Odds Ratio [OR] 34.0, Relative Risk [RR] 4.47, 95% Confidence Interval [CI] 4.904–235.7; p < 0.0001) and CD36 (OR 4.2, RR 1.82, 95% CI 0.9824–17.96; p = 0.04). Moreover, the percentage expression of CD62p (OR 4.0, RR 1.80, 95% CI 0.59–27.24; p = 0.19) was also observed to be probably associated with increased risk of falciparum malaria although not statistically significant (p > 0.05). Conclusion: Plasmodium falciparum malaria has been known to be associated with platelet activation markers, which probably contributes to thrombocytopenia.
Description: An article of published by BioMed Central and also available at https://doi.org/10.1186/s12936-020-03218-4
URI: http://hdl.handle.net/123456789/12306
Appears in Collections:College of Health Sciences

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