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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12313

Title: Diagnosis of bacterial meningitis in Ghana: Polymerase chain reaction versus latex agglutination methods
Authors: Amidu, Nafiu
Antuamwine, Benedict Boateng
Addai-Mensah, Otchere
Abdul-Karim, Abass
Stebleson, Azure
et. al
Issue Date: 17-Jan-2019
Publisher: Public Library of Science
Citation: Amidu N, Antuamwine BB, Addai-Mensah O, Abdul-Karim A, Stebleson A, Abubakari BB, et al. (2019) Diagnosis of bacterial meningitis in Ghana: Polymerase chain reaction versus latex agglutination methods. PLoS ONE 14(1): e0210812. https://doi.org/10.1371/journal. pone.0210812
Abstract: Bacterial meningitis is a public health crisis in the northern part of Ghana, where it contributes to very high mortality and morbidity rates. Early detection of the causative organism will lead to better management and effective treatment. Our aim was to evaluate the diagnostic accuracy of Pastorex and Wellcogen latex agglutination tests for the detection of bacterial meningitis in a resource-limited setting. CSF samples from 330 suspected meningitis patients within the northern zone of Ghana were analysed for bacterial agents at the zonal Public Health Reference Laboratory in Tamale using polymerase chain reaction (PCR) and two latex agglutination test kits; Pastorex and Wellcogen. The overall positivity rate of samples tested for bacterial meningitis was 46.4%. Streptococcus pneumoniae was the most common cause of bacterial meningitis within the sub-region, with positivity rate of 25.2%, 28.2% and 28.8% when diagnosed using Wellcogen, Pastorex and PCR respectively. The Pastorex method was 97.4% sensitive while the Wellcogen technique was 87.6% sensitive. Both techniques however produced the same specificity of 99.4%. Our study revealed that the Pastorex method has a better diagnostic value for bacterial meningitis than the Wellcogen method and should be the method of choice in the absence of PCR.
Description: An article published by Public Library of Science and also available at https://doi.org/10.1371/journal.pone.0210812
URI: http://hdl.handle.net/123456789/12313
Appears in Collections:College of Health Sciences

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