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|Title: ||Plasmodium falciparum histidine-rich protein 2 (PfHRP2) diversity in Ghana|
|Authors: ||Addai-Mensah, Otchere|
Ameke, Selassie Louis
Annani-Akollor, Max Efui
histidine-rich protein 2,
Rapid diagnostic test,
|Issue Date: ||2020|
|Publisher: ||Research Square|
|Abstract: ||Background: In the absence of microscopy, Plasmodium falciparum histidine-rich proteins 2
(PfHRP2)-based rapid diagnostic tests (RDTs) are recommended for the diagnosis of falciparum
malaria, particularly in endemic regions. However, genetic variability of the PfHRP2 gene threatens
the usefulness of the test. This study aimed to investigate the diversity of PfHRP2 in malaria cases
among children in Ghana.
Methods: A cross-sectional study was conducted at the Adidome Government Hospital in the Volta
Region of Ghana. A total of 50 children with mean age of 6.6±3.5 years and diagnosed of falciparum
malaria were included. Blood samples were collected for complete blood count, malaria parasite
identification and counting. DNA samples were amplified and sequenced. Nucleotide sequences were
translated in silico to corresponding amino acids and the deduced amino acids sequences were
analyzed for diversity.
Results: The number of repeats and number of each repeat within PfHRP2 varied between isolates.
Twelve rare PfHRP2 repeat types, two of which are previously unreported, were identified in this
study. Our HRP2 sequence shared high similarity with isolates from Kenya. Using Baker’s regression
model, Group B was the highest occurring type (58.0%). Screening of all sequences for epitopes
recognized by PfHRP2-specific monoclonal antibodies (mAbs), we found the predominant motif to be
AHHAADAHH, which is recognized by the C1-13 mAbs.
Conclusion: This study reports diversity of P. falciparum histidine-rich proteins 2 in samples from
Ghanaian children with symptomatic malaria. We highlight the existence of extra amino acid repeat
types which adds to the PfHRP2 antigenic variability. The findings of this study will contribute to the
understanding of the performance of PfHRP2-based RDTs in the Ghanaian setting.|
|Description: ||An article published by Research Square and also available at 10.21203/rs.3.rs-15776/v1|
|Appears in Collections:||College of Health Sciences|
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