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|Title: ||Molecular-Based Cross-Species Evaluation of Bovine Coronavirus Infection in Cattle, Sheep and Goats in Ghana.|
|Authors: ||Sylverken, A. A|
Lamptey, Jones et al.....
|Keywords: ||Bovine coronavirus|
|Issue Date: ||Apr-2020|
|Publisher: ||Research square|
|Citation: ||Research square|
|Abstract: ||Background: Apart from the huge worldwide economic losses often occasioned by bovine
coronavirus (BCoV) to the livestock industry particularly cattle, continuous surveillance of the virus in
cattle and small ruminants is essential in monitoring variations in the virus that could enhance host
Methods: In this study, we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV
detection was based on reverse transcriptase polymerase chain reaction. Sanger sequencing of the
partial RNA-dependent RNA polymerase (RdRp) region for postive samples were done and nucleotide
sequences were compared with homologous sequences from the GenBank.
Results: The study reports a BCoV prevalence of 0.3% consisting of 4 positive cases; 3 goats and 1
cattle. Bayesian majority-rule cladogram comparing partial and full length BCoV RNA-dependent RNA
polymerase (RdRp) genes obtained in the study to data from the GenBank revealed that the
sequences obtained from this study formed one large monophyletic group with those from different
species and countries. The goat sequences were similar to each other and clustered within the same
clade. No major variations were thus observed with our isolates and those from elsewhere.
Conclusion: Given that Ghana predominantly practice the extensive and semi-intensive systems of
animal rearing, our study highlights the potential for spillover of BCoV to small ruminants in settings
with mixed husbandry and limited separation between species. Continuous surveillance of BCoV in the
country is thus critical to avert future surge and probable increase in host range.|
|Description: ||This article is published in Research square and also available atDOI: 10.21203/rs.3.rs-24727/v1|
|Appears in Collections:||College of Science|
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