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|Title: ||Protein Microarray Analysis of Antibody Responses to Plasmodium falciparum in Western Kenyan Highland Sites with Differing Transmission Levels|
|Authors: ||Baum, Elisabeth|
Molina, Douglas M.
Felgner, Philip L.
|Issue Date: ||2-Dec-2013|
|Publisher: ||Public Library of Science|
|Citation: ||Baum E, Badu K, Molina DM, Liang X, Felgner PL, et al. (2013) Protein Microarray Analysis of Antibody Responses to Plasmodium falciparum in Western Kenyan Highland Sites with Differing Transmission Levels. PLoS ONE 8(12): e82246. doi:10.1371/journal.pone.0082246|
|Abstract: ||Malaria represents a major public health problem in Africa. In the East African highlands, the high-altitude areas were
previously considered too cold to support vector population and parasite transmission, rendering the region
particularly prone to epidemic malaria due to the lack of protective immunity of the population. Since the 1980’s,
frequent malaria epidemics have been reported and these successive outbreaks may have generated some immunity
against Plasmodium falciparum amongst the highland residents. Serological studies reveal indirect evidence of
human exposure to the parasite, and can reliably assess prevalence of exposure and transmission intensity in an
endemic area. However, the vast majority of serological studies of malaria have been, hereto, limited to a small
number of the parasite’s antigens. We surveyed and compared the antibody response profiles of age-stratified sera
from residents of two endemic areas in the western Kenyan highlands with differing malaria transmission intensities,
during two distinct seasons, against 854 polypeptides of P. falciparum using high-throughput proteomic microarray
technology. We identified 107 proteins as serum antibody targets, which were then characterized for their gene
ontology biological process and cellular component of the parasite, and showed significant enrichment for categories
related to immune evasion, pathogenesis and expression on the host’s cell and parasite’s surface. Additionally, we
calculated age-fitted annual seroconversion rates for the immunogenic proteins, and contrasted the age-dependent
antibody acquisition for those antigens between the two sampling sites. We observed highly immunogenic antigens
that produce stable antibody responses from early age in both sites, as well as less immunogenic proteins that
require repeated exposure for stable responses to develop and produce different seroconversion rates between sites.
We propose that a combination of highly and less immunogenic proteins could be used in serological surveys to
detect differences in malaria transmission levels, distinguishing sites of unstable and stable transmission.|
|Description: ||An article published by Public Library of Science and an also available at doi:10.1371/journal.pone.0082246|
|Appears in Collections:||College of Science|
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