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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12526

Title: Dengue virus exposure among blood donors in Ghana
Authors: Narkwa, P. W.
Mutocheluh, M.
Kwofie, T. B.
Owusu, M.
Sylverken, Augustina Angelina
et. al
Keywords: ELISA
RT-PCR
Immunoglobulin
Dengue virus RNA
blood transfusion
Ghana
Issue Date: 2016
Publisher: UDS Publishers Limited
Citation: Journal of Medical and Biomedical Sciences (2016) 5(2): 30-35. doi: http://dx.doi.org/10.4314/jmbs.v5i2.5
Abstract: Dengue is an urban arbovirus whose aetiologic agent is the flavivirus with four distinct antigen serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) that is transmitted to humans through the bite of the mosquito Aedes aegypti. Ghana is endemic for Aedes aegypti mosquitoes and probably dengue viruses. Due to limited data on dengue virus exposure among Ghanaians, we surveyed 188 healthy adult blood donors for the presence of IgG and IgM antibodies to the four serotypes of dengue. Five milliliters of peripheral blood from the blood donors were collected in plain tubes. Serum was then obtained and ELISA tests were employed to detect both dengue virus total antibodies and IgM. The samples were further tested for dengue virus RNA using RT-PCR. Dengue virus IgG was positive for 43.6% of all the 188 blood donor samples tested but all donors were negative for anti-dengue IgM antibody and dengue virus RNA. The rate of dengue virus total antibody exposure did not differ statistically between urban and rural districts. This study shows for the first time that some regions of Ghana are hyperendemic for dengue virus infection but suggests blood for transfusion is invariably dengue virus free. This report has provided a baseline data that will inform wider discussions about the impact of this dengue fever and also guide policy makers to develop effective and affordable early warning and outbreak response systems for Ghana.
Description: An article published by UDS Publishers Limited and also available at doi: http://dx.doi.org/10.4314/jmbs.v5i2.5 INTRODUCTION Dengue fever, dengue haemorrhagic fever and dengue shock syndrome are dangerous and debilitating diseases caused by dengue virus (serotypes 1-4). The World Health Organization (WHO) estimates about 2.5 billion people live in regions potentially at risk of dengue infection. About 100 million cases of dengue fever and more than 500,000 cases of dengue hemorrhagic fever occur on yearly basis while approximately 25,000 die from dengue related clinical manifestations (WHO, 2012). Primary dengue infection has similar clinical picture as malaria. In sub-Saharan Africa, health resource constraints result in the majority of febrile illnesses being presumptively treated as malaria, although growing evidence suggests that in some cases, malaria may only be responsible for a minority of illnesses. Dengue virus belongs to the family Flaviviridae and is transmitted by mosquitoes (Aedes). Flaviviruses are small, lipid-enveloped, positive-stranded RNA viruses (Gubler, 2002). Dengue virus infection in humans is often inapparent (Endy et al., 2011; Simmons et al., 2012) but can lead to a wide range of clinical manifestations, from mild fever to potentially fatal dengue shock syndrome (WHO, 2009). The viremic
URI: http://hdl.handle.net/123456789/12526
Appears in Collections:College of Science

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