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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12562

Title: Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana.
Authors: Burimuah, Vitus
Sylverken, Augustina Angelina
Owusu, Michael
El-Duah, Philip
Yeboah, Richmond
et. al
Keywords: Bovine coronavirus
Cattle
Sheep
Goat
Cross-species infection
Issue Date: 4-May-2020
Publisher: research square
Abstract: Background: Apart from the huge worldwide economic losses often occasioned by bovine coronavirus (BCoV) to the livestock industry particularly cattle, continuous surveillance of the virus in cattle and small ruminants is essential in monitoring variations in the virus that could enhance host switching. Methods: In this study, we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV detection was based on reverse transcriptase polymerase chain reaction. Sanger sequencing of the partial RNA-dependent RNA polymerase (RdRp) region for postive samples were done and nucleotide sequences were compared with homologous sequences from the GenBank. Results: The study reports a BCoV prevalence of 0.3% consisting of 4 positive cases; 3 goats and 1 cattle. Bayesian majority-rule cladogram comparing partial and full length BCoV RNA-dependent RNA polymerase (RdRp) genes obtained in the study to data from the GenBank revealed that the sequences obtained from this study formed one large monophyletic group with those from different species and countries. The goat sequences were similar to each other and clustered within the same clade. No major variations were thus observed with our isolates and those from elsewhere. Conclusion: Given that Ghana predominantly practice the extensive and semi-intensive systems of animal rearing, our study highlights the potential for spillover of BCoV to small ruminants in settings with mixed husbandry and limited separation between species. Continuous surveillance of BCoV in the country is thus critical to avert future surge and probable increase in host range.
Description: An article published by Research Square and also available at 10.21203/rs.3.rs-24727/v1
URI: http://hdl.handle.net/123456789/12562
Appears in Collections:College of Science

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