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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12609

Title: Optimization of protein extraction from fern frond using response surface methodology (RSM)
Authors: Essuman, E. K.
Ankar-Brewoo, Gloria Mathanda
Ofosu, I. W.
Barimah, J.
Keywords: Fern
Nephrolepis biserrata
Arthropteris orientalis
Response Surface Methodology
Agitation Time
Solvents Concentration
Issue Date: 2014
Publisher: Ife Journal of Science
Citation: Essuman et al.: Optimization of Protein Extraction from Fern Frond
Abstract: Protein extractability from Nephrolepis biserrata and Arthropteris orientalis was studied under various conditions. The extraction of fern protein was conducted using alkaline, alcohol and saline treatments under various conditions of treatment concentrations and time of agitation. Central composite design of Response Surface Methodology (RSM) was used for identification of the best condition and extraction yield optimization. Results showed that alkaline treatment produced the highest extraction yield with maximum protein recovery of 5.11 mg/mL and 2.03 mg/mL for Nephrolepis biserrata and Arthropteris orientalis respectively at 0.1 M NaOH concentration and 30 min of agitation time.
Description: An article published by Ife Journal of Science
URI: http://hdl.handle.net/123456789/12609
Appears in Collections:College of Science

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