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|Title: ||Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells|
|Authors: ||Katawa, Gnatoulma|
Layland, Laura E.
Debrah, Alex Y.
Horn, Charlotte von
|Issue Date: ||8-Jan-2015|
|Publisher: ||PLoS Negl Trop Dis|
|Citation: ||Katawa G, Layland LE, Debrah AY, von Horn C, Batsa L, et al. (2015) Hyperreactive Onchocerciasis is Characterized by a Combination of Th17-Th2Immune Responses and Reduced Regulatory T Cells. PLoS Negl Trop Dis 9(1): e3414. doi:10.1371/journal.pntd.0003414|
|Abstract: ||Clinical manifestations in onchocerciasis range from generalized onchocerciasis (GEO) to the rare but severe hyperreactive(HO)/sowda form. Since disease pathogenesis is associated with host inflammatory reactions, we investigated whether Th17responses could be related to aggravated pathology in HO. Using flow cytometry, filarial-specific cytokine responses andPCR arrays, we compared the immune cell profiles, including Th subsets, in individuals presenting the two polar forms ofinfection and endemic normals (EN). In addition to elevated frequencies of memory CD4+T cells, individuals with HOshowed accentuated Th17 and Th2 profiles but decreased CD4+CD25hiFoxp3+regulatory T cells. These profiles includedincreased IL-17A+, IL-4+, RORC2+and GATA3+CD4+T cell populations. Flow cytometry data was further confirmed using aPCR array since Th17-related genes (IL-17 family members, IL-6, IL-1band IL-22) and Th2-related (IL-4, IL-13, STAT6) geneswere all significantly up-regulated in HO individuals. In addition, strongerOnchocerca volvulus-specific Th2 responses,especially IL-13, were observedin vitroin hyperreactive individuals when compared to GEO or EN groups. This studyprovides initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating thedevelopment of severe onchocerciasis.|
|Description: ||An article published by PLoS Negl Trop Dis and also available at doi:10.1371/journal.pntd.0003414|
|Appears in Collections:||College of Science|
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