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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/13093

Title: EVALUATION OF THE ANTI - PROLIFERATIVE EFFECT, ANTIOXIDANT AND PHYTOCHEMICAL CONSTITUENTS OF FICUS PUMILA LINN
Authors: Larbie, Christopher
Torkornoo, Dennis
Agbenyegah, Sefakor
Dowuona, Jasmine Naa Norkor
Appiah-Oppong, Regina
Dotse, Eunice
Aning, Abigail
Adu, Bright
Sinclear, Caleb
Keywords: Ficus pumila
Phytochemicals
Cytotoxicity
Antioxidant
Cancer
Selectivity
Issue Date: 1-May-2019
Publisher: International Journal of Pharmaceutical Sciences and Research
Citation: International Journal of Pharmaceutical Sciences and Research,Vol. 10(5): 2605-2618.
Abstract: This study sought to evaluate the heavy metal content of the raw powder and extract of the plant, the phytochemical constituents, antioxidant effect by the use of DPPH assay, the total phenolic content using Folin Ciocalteu assay and the cytotoxic effect using the MTT Assay of Ficus pumila ethanolic extract, methanolic and hydro fractions on liver cancer cells (HepG2), Leukemic cells (Jurkat) and normal liver cells (Chang). FTIR and Gas Chromatography-Mass Spectrometry (GC-MS) was used to identify the functional groups and major constituents of the most active fraction of F. pumila. Alkaloids, terpenoids, flavonoids, cardiac glycoside, saponins, and tannins were present in the ethanolic extract of F. pumila. The heavy metal analysis revealed the presence of Iron in both the raw powder (1.97 ± 0.11 mg/l) and extract (0.92 ± 0.02 mg/L). Zinc was also detected in both the raw powder (1.19 ± 0.00 mg/l) and extract (0.6595 ± 0.02 mg/l). The results from the FTIR revealed the presence of alkynes, alkyl halides, aromatics and aliphatic amines common to all fractions and compounds such as Phenol, 2,4-bis (1,1-Dimethylethyl) and Dodecane, 2,6, 10-trimethyl were detected in the samples by GC-MS. The DPPH assay also showed that all the fractions scavenged DPPH free radical in a dose-dependent manner as compared to the positive control (Ascorbic acid) and positively correlated to the phenolic contents. The MTT assay revealed that methanolic fraction was selective towards the Jurkat cell lines (Selectivity Index = 2.822). This increases the prospects that this plant contains compound(s) which could serve as leads for novel anticancer drugs.
Description: This article has been published by International Journal of Pharmaceutical Sciences and Research and is available at http://dx.doi.org/10.13040/IJPSR.0975-8232.10(5).2605-18
URI: 10.13040/IJPSR.0975-8232.10(5).2605-18
http://hdl.handle.net/123456789/13093
Appears in Collections:College of Science

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