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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/13103

Title: Methoxy-flavones identified from Ageratum conyzoides induce capase -3 and -7 activations in Jurkat cells
Authors: Larbie, Christopher et al.,
Acheampong, Felix
Reilly, John
Spencer, Matthew
Gunderson, Karl
Appiah-Opong, Regina
Howson, Christopher
Porier, Jennifer
Joyce, Kellie
Jeliazkova, Valentina
Voytek, Sarah
Ginsburg-Moraff, Carol
Thibodeaux, Stefan
Kublbeck, Jill
Austin, Steven
Keywords: Ageratum conyzoides
methoxy-flavones
Jurkat
biomimetic affinity chromatography
cell viability
Issue Date: 10-Oct-2017
Publisher: Journal of Medicinal Plants Research
Citation: Journal of Medicinal Plants Research,Vol. 11(38), pp. 583-590,
Abstract: New therapies for leukemia are urgently needed due to adverse side effects, tumor resistance and lack of selectivity of many chemotherapeutic agents in clinical use. Ageratum conyzoides has been used in folklore medicine for managing leukemia and other cancers. Thus, this study aimed to investigate the effects of fractions, sub-fractions and purified compounds from the ethanol leaf extracts of A. conyzoides against Jurkat cells-model for acute T cell leukemia. A two-dimensional purification process using normal phase flash, followed by reverse phase purification was necessary to isolate pure methoxy-flavones, which were further characterized by Nuclear Magnetic Resonance (NMR) and MS-MS. The effect of fractions or pure compounds on cell viability was determined using either the MTT reagent or CellTiter-Blue® assay, while the caspase-3 and -7 activation was tested with Caspase-Glo® 3/7 assay. Prediction of compounds’ drug disposition profiles in vivo was measured with biomimetic affinity chromatography methodologies.
Description: This article has been published by Journal of Medicinal Plants Research and is also available at DOI: 10.5897/JMPR2017.6457
URI: 0.5897/JMPR2017.6457
http://hdl.handle.net/123456789/13103
Appears in Collections:College of Science

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