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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1360

Title: Formulation of dehydrated media from the bean mucilage and placenta of mature unripe cocoa pods for-culturing Phytophthora palmivora (Butt.) But!
Authors: Frimpong, M.
Awuah, R. T.
Issue Date: May-2003
Publisher: Ghana Journal Agricultural Science
Citation: Ghana Journal Agricultural Science, vol. 36, pg. 97-103
Abstract: Because of difficulty in obtaining cocoa pods during the off-cocoa growing season to prepare media from scratch, formulation of dehydrated media from the placenta and wed mucilage of green mature, cocoa pods was attempted for use in culturing phytophihora palmivora. Pieces of mucilage and placenta were oven-dried (70 C. 6 h), milled and the resulting 1lakcs sieved to obtain a fine powder. Four powder concentrations 0, 1, 2 and 3 per cent in water (wIy) were prepared, and agar and calcium carbonate added at 2 per cent (w/v) and 2.5 per cent (w/w), respectively,-to each powder concentration. The media rerc autoclaved and evaluated in Petri plates for clarity and for capaciay in support growth and sporulation of Phylophthoru pulaivuru. Powder yield was higher for the placenta than lire the mucilage-based media. The source of powder, powder concentration, and their interaction all had significant effects on radial niycclial growth of P palmivora. At the same powder concentration, media prepared from the placenta supported significantly higher (P 5 0.05) radial growth of the test fungus (5.59-7.10 min/day) than media prepared from the mucilage powder, (4.54 - 6.18 min/day). Aerial mycclial growth improved slightly as powder concentration increased but media' clarity decreased. Sporulation of P. palmivora was good on all the media. Powder source, powder concentration, and method of media preparation before autoclaving all had significant effects on radial fungal growth..At each concentration and for each type of powder, growth was faster when the medium was boiled before autoclaving. Media incorporating 2 per cent placenta powder, 2.5 per cent calcium carbonate, and'2 per cent agar prepared by boiling and filtering' with cheese cloth before autoclaving had the best result and are recommended for culturing P palmivora. This dehydrated medium is easy to prepare, has a pH of about 7. and can be stored for a long time without caking.
Description: This article was published by Ghana Journal Agricultural Science,2003 vol. 36, pg. 97-103.
URI: http://hdl.handle.net/123456789/1360
Appears in Collections:College of Health Sciences

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