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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1365

Title: Vertical Transmission of HIV in Ghanaian Women Diagnosed in Cord Blood and Post-Natal Samples
Authors: Fischetti, Lucia
Danso, Kwabena
Dompreh, Albert
Addo, Victor
Haaheim, Lars
Allain, Jean-Pierre
Keywords: HIV
Vertical transmission
Cord blood
Viral load
Issue Date: 2005
Publisher: Wiley-Liss
Abstract: HIV RNA detection in the newborn is the main diagnostic tool for vertical transmission. Most infections are thought to occur park or post- natally, hence preventive antiviral therapy admi- nistered days before and during delivery. This study used cord blood for molecular diagnosis, examined viral load and HIV-1 subtypes as deter- minants of transmission, and compared molec- ular variability of maternal, cord blood, and post-natal quasispecies. Ninety-seven seroposi- tive mother-cord blood paired plasmas from Ghana.were tested for HIV RNA. Viral load was quantified and a subgroup of 45 random women samples was typed and subtyped. HIV-1 from infected pairs was cloned, sequenced, and anal- yzed phylogenetically. The prevalence of HIV infection in pregnant women was 3.3%. 13/97 cord blood samples (13.5%) contained HIV RNA. No correlation between either viral load at labor (range 103-107) or HIV-1 subtype and in utero transmission was found. In both transmitting and non-transmitting mothers, 56% of HIV-1 strains were CRF02_AG. In three pairs, maternal and cord blood quasispecies were closely related, suggesting late pregnancy or perinatal transmission, while in four pairs, genetic distances suggested transmission earlier during gestation. Maternal viral load and genotype did not correlate with HIV-1 pre-natal transmission. HIV infection during gestation appears relatively frequent.
Description: This article was published by Lucia Fischetti...[et.al]
URI: http://hdl.handle.net/123456789/1365
Appears in Collections:College of Health Sciences

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