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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/14428

Title: Prevalence and risk factors of cryptosporidium infection among children with diarrhoea reporting at the Presbyterian Hospital, Agogo, Ghana
Authors: Oppong, Kwabena
Maigaascofare, Oumou
Keywords: Risk Factors
Cryptosporidium
Children with Diarrhoea
Presbyterian Hospital
Agogo
Ghana
Issue Date: 19-Jul-2021
Abstract: Cryptosporidium, an enteric coccidian pathogen, causes diarrhoea in children and adults worldwide. In immunocompetent persons, Cryptosporidium infection (cryptosporidiosis) is often self-limiting but can be severe and life threatening in infants and immune-suppressed patients; however, the disease is under-recognised. This cross-sectional study aimed to estimate the prevalence and assess the risk factors associated with cryptosporidiosis among children who reported at the OPD of Agogo Presbyterian hospital (APH) with diarrhoea from January to December 2017. A total of 204 stool samples were collected from these diarrhoea patients with the consent of their parents or guardians and tested for Cryptosporidium using rapid diagnostic test kit (RDT) and polymerase chain reaction (PCR). Through follow-ups on patients whose samples tested positive for Cryptosporidium (Index cases), 58 stool samples were collected from household members, 78 from neighbours, and 92 from animals. Real time PCR, restriction fragment length polymorphism (RFLP) PCR, and 60KDa glycoprotein (gp60) PCR assays were employed to identify and characterise the Cryptosporidium species isolated. A prevalence of 9.80% was recorded from the index cases based on the RDT results. Among household members and neighbours of the index cases, the highest prevalence recorded was 8.62% and 21.80%, respectively and 21.74% among animals based on the PCR-RFLP results. From the genomic sequence analysis of species isolated, 3 (6.98%) of the isolates were classified as C. felis, 14 (32.56%) as C. hominis, 1 (2.33%) as C. meleagridis, 11 (25.58%) as C. parvum, and 14 (32.56%) as C. xiaoi. With the exception of C. xiaoi, which was found in only animals, all the other Cryptosporidium species were found in humans. To assess the relationships between Cryptosporidium infection and socio-demographic characteristics, which have suggestively been implicated in the transmission of the disease, Fischer’s exact test and Pearson’s Chi square test was used to assess the significance of association between some socio-demographic characteristics and cryptosporidiosis. However, no significant association was observed between these risk factors and cryptosporidiosis. In conclusion, the prevalence of Cryptosporidium was found to be 9.80% among children who reported to the OPD of APH with diarrhoea. Testing for Cryptosporidium is therefore recommended to be part of the routine diagnostic tests performed for children and other immunocompromised patients who report to the hospital with diarrhoea.
Description: A thesis submitted in partial fulfillment of the requirements for the Degree of Master of Philosophy in the Department of Clinical Microbiology, School of Medicine and Dentistry, College Of Health Sciences. June, 2019
URI: http://hdl.handle.net/123456789/14428
Appears in Collections:College of Health Sciences

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