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Title: | Molecular Characterization of Glucose-6-Phosphate Dehydrogenase: Do Single Nucleotide Polymorphisms Affect Hematological Parameters in HIV-Positive Patients |
Authors: | Danquah, Kwabena Owusu Mensah, Kofi Nkansah, Charles Appiah, Samuel Kwasi Noagbe, Mark Hardy, Yasmine Ntiamoah, David O. Boateng, Lillian Antwi Annani-Akollor, Max Efui Owiredu, Eddie-Williams Debrah, Alexander Yaw Addai-Mensah, Otchere |
Issue Date: | Aug-2021 |
Publisher: | Journal of Tropical Medicine |
Citation: | Journal of Tropical Medicine, Volume 2020, Article ID 5194287, 7 pages |
Abstract: | .is descriptive, cross-sectional study aimed at evaluating the prevalence of G6PD deficiency and the 376A⟶G, 202G⟶A single
nucleotide polymorphisms (SNPs) among HIV patients attending care at a teaching hospital in Ghana and determine how the SNPs
affect haematological profile in HIV. A total of 200 HIV-positive Ghanaians were recruited. Venous blood samples were obtained and
complete blood count, and G6PD screening and genotyping for the 376A⟶G, 202G⟶A SNPs were performed. Out of the 200
participants, 13.0% (26/200) were G6PD-deficient based on the methemoglobin reductase technique, with 1.5% (3/200) and 11.5% (23/
200) presenting with partial and full enzyme defect, respectively. Among the 13.0% participants with G6PD deficiency, 19.2% (5/26),
30.8% (8/26), and 19.2% (5/26) presented with 376A⟶G only (enzyme activity (EA): 1.19 U/g Hb), 202G⟶A only (EA: 1.41 U/g
Hb), and G202/A376 SNPs (EA: 1.14 U/g Hb), respectively. Having the 376A⟶G mutation was associated not only with lower red
blood cell (RBC) count (3.38 ×106/μL (3.16–3.46) vs 3.95 ×106/μL (3.53–4.41), p � 0.010) but also with higher mean cell volume (MCV)
(102.90 (99.40–113.0) vs 91.10 fL (84.65–98.98), p � 0.041) and mean cell haemoglobin (MCH) (33.70 pg (32.70–38.50) vs 30.75 pg
(28.50–33.35), p � 0.038), whereas possessing the 202G⟶A mutation was associated with higherMCVonly (98.90 fL (90.95–102.35)
vs 91.10 fL (84.65–98.98), p � 0.041) compared to G6PD nondeficient participants. .e prevalence of G6PD deficiency among HIV
patients in Kumasi, Ghana, is 13.0% prevalence, comprising 1.5% and 11.5% partial and full enzyme defect, respectively, based on the
methemoglobin reductase technique among HIV patients in Ghana. Among G6PD-deficient HIV patients, the prevalence of G202/
A376 SNPs is 19.2%. .e 376A⟶G mutation is associated not only with lower RBC count but also with higher MCV and MCH,
whereas the 202G⟶A mutation is associated with higher MCV compared to the normal G6PD population |
Description: | This article is published by Journal of Tropical Medicine and is also available at https://doi.org/10.1155/2020/5194287 |
URI: | 10.1155/2020/5194287 http://hdl.handle.net/123456789/14948 |
Appears in Collections: | College of Health Sciences
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