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Title: | Molecular-based cross-species evaluation of bovine coronavirus infection in cattle, sheep and goats in Ghana |
Authors: | Burimuah, Vitus Sylverken, Augustina Angelina Owusu, Michael El-Duah, Philip Yeboah, Richmond Lamptey, Jones Frimpong, Yaw Oppong Agbenyega, Olivia Folitse, Raphael Emikpe, Ben Tasiame, William Owiredu, Eddie-Williams Oppong, Samuel Antwi, Christopher Adu-Sarkodie, Yaw Drosten, Christian |
Keywords: | Bovine coronavirus Cattle Sheep Goat Cross-species infection |
Issue Date: | 1-Mar-2022 |
Abstract: | Background: Apart from the huge worldwide economic losses often occasioned by bovine coronavirus (BCoV) to
the livestock industry, particularly with respect to cattle rearing, continuous surveillance of the virus in cattle and
small ruminants is essential in monitoring variations in the virus that could enhance host switching. In this study,
we collected rectal swabs from a total of 1,498 cattle, sheep and goats. BCoV detection was based on reverse
transcriptase polymerase chain reaction. Sanger sequencing of the partial RNA-dependent RNA polymerase (RdRp)
region for postive samples were done and nucleotide sequences were compared with homologous sequences
from the GenBank.
Results: The study reports a BCoV prevalence of 0.3%, consisting of 4 positive cases; 3 goats and 1 cattle. Less than
10% of all the animals sampled showed clinical signs such as diarrhea and respiratory distress except for high
temperature which occurred in > 1000 of the animals. However, none of the 4 BCoV positive animals manifested
any clinical signs of the infection at the time of sample collection. Bayesian majority-rule cladogram comparing
partial and full length BCoV RdRp genes obtained in the study to data from the GenBank revealed that the
sequences obtained from this study formed one large monophyletic group with those from different species and
countries. The goat sequences were similar to each other and clustered within the same clade. No major variations
were thus observed between our isolates and those from elsewhere. Conclusions: Given that Ghana predominantly practices the extensive and semi-intensive systems of animal
rearing, our study highlights the potential for spillover of BCoV to small ruminants in settings with mixed husbandry
and limited separation between species. |
URI: | http://hdl.handle.net/123456789/15582 |
Appears in Collections: | College of Health Sciences
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