Conference Proceedings >
College of Health Sciences >
Please use this identifier to cite or link to this item:
|Title: ||blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana|
|Authors: ||Agbenyega, Edward Tsiri|
Partridge, Sally R.
Iredell, Jonathan R.
|Issue Date: ||9-Sep-2015|
|Publisher: ||Elsevier Ltd|
|Citation: ||Elsevier Ltd, 2015|
|Abstract: ||Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases (ESBLs) are among the
most multidrug-resistant pathogens in hospitals and are spreading worldwide. Horizontal gene transfer and
spread of high-risk clones are involved in ESBL dissemination. Investigation of the resistance phenotypes of
101 consecutive clinical E. coli (n=58) and K. pneumoniae (n=43) isolated at the Komfo Anokye Teaching Hospital
inGhana over 3months revealed 63 (62%)with an ESBL phenotype. All 63 had a blaCTX-Mgene, and sequence
analysis showed that 62 of thesewere blaCTX-M-15. blaCTX-M-15was linked to ISEcp1 and orf477Δ in all isolates, and
most isolates also carried blaTEM, aac(3)-II, aacA4cr, and/or blaOXA-30 genes on IncF plasmids. XbaI/pulsed-field
electrophoresis showed heterogeneity among isolates of both species, suggesting that blaCTX-M-15 dissemination
is caused by horizontal gene transfer rather than clonal spread of these species in Ghana.|
|Description: ||This article is published at Elsevier Ltd, and also available at http://dx.doi.org/10.1016/j.diagmicrobio.2015.12.010|
|Appears in Collections:||College of Health Sciences|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.