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Title: | blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana |
Authors: | Agbenyega, Edward Tsiri Agyekum, Alex Fajardo-Lubián, Alicia Ansong, Daniel Partridge, Sally R. Iredell, Jonathan R. |
Keywords: | PFGE Enterobacteriaceae Antibiotic resistance ESBLs CTX-M enzymes Ghana |
Issue Date: | 9-Sep-2015 |
Publisher: | Elsevier Ltd |
Citation: | Elsevier Ltd, 2015 |
Abstract: | Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases (ESBLs) are among the
most multidrug-resistant pathogens in hospitals and are spreading worldwide. Horizontal gene transfer and
spread of high-risk clones are involved in ESBL dissemination. Investigation of the resistance phenotypes of
101 consecutive clinical E. coli (n=58) and K. pneumoniae (n=43) isolated at the Komfo Anokye Teaching Hospital
inGhana over 3months revealed 63 (62%)with an ESBL phenotype. All 63 had a blaCTX-Mgene, and sequence
analysis showed that 62 of thesewere blaCTX-M-15. blaCTX-M-15was linked to ISEcp1 and orf477Δ in all isolates, and
most isolates also carried blaTEM, aac(3)-II, aacA4cr, and/or blaOXA-30 genes on IncF plasmids. XbaI/pulsed-field
electrophoresis showed heterogeneity among isolates of both species, suggesting that blaCTX-M-15 dissemination
is caused by horizontal gene transfer rather than clonal spread of these species in Ghana. |
Description: | This article is published at Elsevier Ltd, and also available at http://dx.doi.org/10.1016/j.diagmicrobio.2015.12.010 |
URI: | http://dx.doi.org/10.1016/j.diagmicrobio.2015.12.010 http://hdl.handle.net/123456789/15678 |
Appears in Collections: | College of Health Sciences
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