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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/15955

Title: Development and characterisation of chitosan films impregnated with insulin loaded PEG-b-PLA nanoparticles (NPs): A potential approach for buccal delivery of macromolecules
Authors: Giovino, Concetta
Ayensu, Isaac
Tetteh, John
Boateng, Joshua S.
Keywords: Buccal delivery
Chitosan films
Poly(ethylene glycol)methyl
Issue Date: Mar-2012
Publisher: International Journal of Pharmaceutics 428 (2012) 143– 151
Citation: International Journal of Pharmaceutics 428 (2012) 143– 151
Abstract: Mucoadhesive chitosan based films, incorporated with insulin loaded nanoparticles (NPs) made of poly(ethylene glycol)methyl ether-block-polylactide (PEG-b-PLA) have been developed and characterised. Blank-NPs were prepared by double emulsion solvent evaporation technique with varying concentrations of the copolymer (5 and 10%, w/v). The optimised formulation was loaded with insulin (model protein) at initial loadings of 2, 5 and 10% with respect to copolymer weight. The developed NPs were analysed for size, size distribution, surface charge, morphology, encapsulation efficiency and drug release. NPs showing negative ( )-potential (<−6 mV) with average diameter > 300 nm and a polydispersity index (P.I.) of ≈0.2, irrespective of formulation process, were achieved. Insulin encapsulation efficiencies of 70% and 30% for NPs-Insulin-2 and NPs-Insulin-5 were obtained, respectively. The in vitro release behaviour of both formulations showed a classic biphasic sustained release of protein over 5 weeks which was influenced by pH of the release medium. Optimised chitosan films embedded with 3 mg of insulin loaded NPs were produced by solvent casting with homogeneous distribution of NPs in the mucoadhesive matrix, which displayed excellent physico-mechanical properties. The drug delivery system has been designed as a novel platform for potential buccal delivery of macromolecules.
Description: This article is published by International Journal of Pharmaceutics and is also available at doi:10.1016/j.ijpharm.2012.02.035
URI: 10.1016/j.ijpharm.2012.02.035
Appears in Collections:College of Health Sciences

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