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|Title: ||Design of an HPLC method for the assay of a multicomponent cold preparation (Ascorbic Acid), Paracetamol, and Caffeine)|
|Authors: ||Acheampong, Joseph Kofi|
|Issue Date: ||14-Dec-2001|
|Series/Report no.: ||3050;|
|Abstract: ||A rapid reversed-phase High Performance Liquid Chromatographic (HPLC) method was developed for the simultaneous assay of Ascorbic acid, Paracetamol, and Caffeine in a tablet matrix. The powdered sample dissolved in 0.04% w/v Na2S2O3 solution containing Benzoic acid as the internal standard was injected into the chromatograph. Ascorbic acid exhibited stability in the 0.04 %w/v Na2S2O3 solution for five (5) days.
The HPLC system used Aquapore RP-300 column, methanol- sodium dihydrogen phosphate (17:83, pH 4.25 ± 0.05) as the mobile phase, The isocratic form of elution was employed at a flow rate of 1.5 ml/min. Chromatography was carried out at ambient temperature with total chromatographic time of approximately 8 minutes. Detection was done at 273nm and 0.3 AUFS. The sensitivity for the analysis was 0.51 μg/ ml for Ascorbic acid, 5 μg/ml for Paracetamol, and 3 μg/ml for Caffeine.
Quantification was based on the drug- internal standard peak area ratio. This ratio was linear over a concentration range of l0-100 μg/ml of Ascorbic acid, l00-l000 μg/ml of Paracetamol, and 6-60 μg/m1 of Caffeine. Overall label claim (± SD) from four commercial tablets were Ascorbic acid, 97.03 ± 0.73%; Paracetamol, 99.80 ± 1.40%; Caffeine, 95.03 ± 0.90%.
The method was compared to the standard UV spectrophotometric method and also applied to the assay of commercial tablets containing these three active ingredients. The HPLC method is applicable to the assay of individual tablets. The HPLC method was found to be faster, convenient and more precise.|
|Description: ||A thesis submitted to the Department of Pharmaceutical Chemistry, College of Health Sciences,
Kwame Nkrumah University of Science and Technology, in partial
fulfilment of the requirements for the award of Master of Science degree, 2001|
|Appears in Collections:||College of Health Sciences|
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