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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/5063

Title: NMR LIPID Analysis of Platelet Membranes of Patients with Coronary Artery Diseases: Case Studies
Authors: Yeboah, F.A
Gibbons, W.A.
Preedy, V.
Keywords: proton nuclear magnetic resonance (Nmr)
coronary heart disease
Issue Date: 2000
Publisher: Journal of Science and Technology
Citation: Journal of Science and Technology, Vol. 20, Nos 1,2 & 3 2000. pp 139-144
Abstract: A high resolution proton nuclear magnetic resonance (NMR) was used to investigate the platelet membrane lipid composition of subjects who have been definitively diagnosed as having various forms of coronary heart disease (CAD). The one dimensional (ID) proton spectra were re¬corded at a temperature of 29S°K in Fourier transform(FT) mode with 16K data points, using a 45" detection pulse and 2.0 acquisition time with a solvent presaturation during relaxation to remove excess water signal. The major phospholipids and cholesterol present were identified and estimated and compared to those of normal individuals' platelet membranes. Platelet cholesterol content as analysed by the proton Nmr in all the 3 patients investigated were significantly elevated (p<(). ()()()()I). In contrast, arachidonic acid levels in the three patients were significantly reduced (p<0.0002) as well as phosphatidylcholine (p<0.040S) and phosphatidylethanolamine (p<0.0l6). The degree of unsaturation in the various structural phospholipids were also compared and was found to be less than those of the control subjects. Cholesterol: phospholipid ratio were also raised in the patients (p<0.0016). This work has shown that vital clinical information could be derived from lipid analysts by proton nuclear resonance which could be of clinical significance in the management of such patients.
Description: Article published in Journal of Science and Technology, Vol. 20, Nos 1,2 & 3 2000. pp 139-144
URI: http://hdl.handle.net/123456789/5063
Appears in Collections:Journal of Science and Technology 2000-

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