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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/5165

Title: Molecular Response of Atlantic Cod’s (Gadus Morhua L.) Cypia, Prolactin and Zona Radiata Genes upon Exposure to Polychlorinated Biphenyls
Authors: Addo, M.G.
Akanwariwiak, W. G.
Male, R.
Keywords: Cytochrome P450IA
Biomarker
Gene expression
Issue Date: Aug-2007
Publisher: Journal of Science and Technology
Citation: Journal of Science and Technology, Vol. 27 No. 2, 2007 pp 32-40
Abstract: A molecular response study was conducted to determine whether Polychlorinated Biphenyl or PCB (Cloplien A40) had an effect on the induction of the zona radiata, prolactin and cytochrome P4501A (CYPIA) mRNAs in matured Atlantic cod (Gadus morhua). A total of 127 fishes were maintained under natural photoperiod in tanks and fed with wet pellets containing Cloplien A40. RNA samples were taken from the gonads, pituitary and the liver from day 0, 48 and 105 after sacrificing the fish and stored at -20°C prior to analysis. Analysis of gene induction by Northern hybridization assay showed the induction of prolactin and CYPIA in the pituitary and the liver tissues respectively after PCB treatment. The induction was found to be sex, age and seasonal specific. Males had greater gene expression than the females. There was however, no observed difference between the controlled and exposed fishes in terms of zona radiata gene expression. Although the picture is far from complete, the findings demonstrate the potential of using gene induction as a biomarker of aquatic pollution.
Description: Article published in the Journal of Science and Technology, Vol. 27 No. 2, 2007 pp 32-40
URI: http://hdl.handle.net/123456789/5165
Appears in Collections:Journal of Science and Technology 2000-

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