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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/5272

Title: Quality Evaluation of Paracetamol in the Bulk, Dosage Forms and Body Fluids Using the High Performance Liquid Chromatography (HPLC) Technique
Authors: Asare-Nkansah, S.
Kwakye, J.K.
Keywords: Chromatography
internal standard
in vivo and in vitro
Issue Date: Apr-2006
Publisher: Journal of Science and Technology
Citation: Journal of Science and Technology, Vol. 26 No. 1, 2006 pp 22-31
Abstract: High Performance Liquid Chromatography has been used to evolve an analytical procedure for the evaluation of the content of paracetamol in the bulk, dosage forms and in urine, a body fluid. Separation and resolution have been achieved with a combination of methanol and 2.5% acetic acid (15:85) on a reversed-phase column at ambient temperature. Elution was isocratic with UV detection at 257nm. Internal standard calibration method was used for quantitation with caffeine as the internal standard. Mean retention times for paracetamol and caffeine were respectively 2.61 ± 0.13 win and 11.98 ± 0.72 min. The calibration curve was linear over the range 0.1-5.Og/ml. The method was also suitable for the assay of paracetamol-codeine combination drug as well as estimation of the amount of constituents in urine when the wavelength of UV detection was 245 nm with acetanilide as the internal standard.
Description: Article published in the Journal of Science and Technology, Vol. 26 No. 1, 2006 pp 22-31
URI: http://hdl.handle.net/123456789/5272
ISSN: 08550395
Appears in Collections:Journal of Science and Technology 2000-

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