Characterisation of Jasmine 85 rice (Oryza sativa) variety from different sources of seed production in Ghana

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2014-10-20
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Following the observation that rice variety Jasmine 85 from two sources in Ghana were different in morphology, samples of Jasmine 85 were collected from seven sources in Ghana. A reference source was obtained from Africa Rice Centre in Senegal for comparison. An experiment was set up in a Randomized Complete Block Design and under standard conditions in Nobewam (Ashanti region) to ascertain if there were any differences among the sources. Morphological data was taken in the field to identify off types and characterise the sources. All the sources were not significantly different in terms of aroma, anthocyanin coloration, leaf pubescence, and ligule shape, but the sources showed significant differences with regards to pericarp colour, days to 50% heading, plant height, number of tillers, seed length, seed width, panicle length, and number of secondary branches. Physico-chemical analyses were done for further characterisation. Grain size and shape, grain chalkiness, cooking time, head rice yield, gelatinisation temperature, amylose content, and viscosity properties were significantly different among the sources. Grain hardness was not significantly different among the sources. Molecular characterisation using 15 SSR markers was done to establish the genetic resemblance among the sources. The sources differed significantly, although the result also showed that they are closely related. A cluster analysis run on the morphological and physico-chemical data gave four clusters: (GBEWAA and TONO), (SARI), (PV), (KIP, CRI, ARI, DARTEY). The molecular data on the other hand gave three clusters: (TONO, DARTEY, PV), (SARI, ARI, CRI), (KIP, GBEWAA). The results imply that seeds from different sources should not be mixed for production, and these varieties should be treated separately in future evaluations.
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A thesis submitted to the School of Research and Graduate Studies, Kwame Nkrumah University of Science and Technology, in partial fulfillment of the requrememts for the award of Master of Philosophy (Seed Science and Technology) degree.
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