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|Title: ||Fluorescence in situ hybridization (fish) for rapid identiﬁcation of salmonella spp. from agar and blood culture broth—an option for the tropics?|
|Authors: ||Frickmann, Hagen|
Norbert, , G. Schwarz
Hagen, Ralf M
Fluorecensce in situ hybridization
|Issue Date: ||2013|
|Publisher: ||International Journal of Medical Microbiology|
|Citation: ||International Journal of Medical Microbiology Vol. 303,issue 5 (2013) 277–284|
|Abstract: ||Background: Salmonella enterica is an important cause of diarrhea with the potential to cause systemic infection including sepsis, particularly in the tropics. Sepsis in particular requires quick and reliable identiﬁcation to allow a rapid optimization of antibiotic therapy. We describe the establishment and eval- uation of ﬂuorescence in situ hybridization (FISH) as a rapid and easy-to-perform molecular identiﬁcation procedure from agar and blood culture broths.
Methods: Two newly developed FISH probes with speciﬁcity for Salmonella spp. were evaluated with 10 reference strains, 448 clinical isolates of Gram-negative bacteria from Germany and Ghana including 316
Salmonella spp. strains, and 39 environmental Salmonella spp. isolates from rivers and streams in Ghana. One FISH probe was further tested with 207 pre-incubated blood culture broths from Germany with Gram-negative rod-shaped bacteria in Gram stain.
Results: Evaluation of the newly designed FISH probes demonstrated sensitivity of 99.2% and speciﬁcity of
98.4% for clinical isolates, sensitivity of 97.4% for environmental Salmonella spp. isolates, and sensitivity of 100% and speciﬁcity of 99.5% for blood culture materials.
Conclusions: FISH proved to be highly reliable for a rapid identiﬁcation of Salmonella spp. directly from pre-incubated blood culture broths as well as after growth on agar. The inexpensive and easy-to-perform procedure is particularly suitable for resource-limited areas where more sophisticated procedures are
|Description: ||Article published in International Journal of Medical Microbiology Vol. 303,issue 5 (2013) 277–284. Doi:10.1016/ji.ijmm2013.04.00
Also available at: www.elsevier.co m/locate/ijmm|
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