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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/7248

Title: Determination of Triacylglycerol Regioisomers using Electrospray Ionization-Quadrupole Ion Trap Mass Spectrometry with a Kinetic Method
Authors: Leveque, Nathalie L.
Acheampong, Akwasi
Heron, Sylvie
Tchapla, Alain
Keywords: Kinetic method
Mass spectrometry
Regiospecificity
Triacylglycerols
Issue Date: 2012
Publisher: Analytica Chimica Acta
Citation: Analytica Chimica Acta 722 (2012) 80– 86
Abstract: The kinetic method was applied to differentiate and quantify mixtures of regioisomeric triacylglycerols (TAGs) by generating and mass selecting alkali ion bound metal dimeric clusters with a TAG chosen as reference (ref) and examining their competitive dissociations in a quadrupole ion trap mass spectrometer. This methodology readily distinguished pairs of regioisomers (AAB/ABA) such as LLO/LOL, OOP/OPO and SSP/SPS and consequently distinguished sn-1/sn-3, sn-2 substituents on the glycerol backbone. The dimeric complex ions [ref, Li, TAG(AAB and/or ABA)]+ generated by electrospray ionization mass spectrometry were subjected to collision induced dissociation causing competitive loss of either the neutral TAG reference (ref) leading to [Li(AAB and/or ABA)]+ or the neutral TAG molecule (TAG(AAB and/or ABA)) leading to [ref, Li]+. The ratio of the two competitive dissociation rates, defined by the product ion branching ratio (Riso), was related via the kinetic method to the regioisomeric composition of the investigated TAG mixture. In this work, a linear correlation was established between composition of the mixture of each TAG regioisomer and the logarithm of the branching ratio for competitive fragmentation. Depending on the availability of at least one TAG regioisomer as standard, the kinetic method and the standard additions method led to the quantitative analysis of natural TAG mixtures.
Description: Article published in Analytica Chimica Acta 722, 2012. Also available at www.elsevier.com/locate/aca
URI: http://hdl.handle.net/123456789/7248
Appears in Collections:College of Science

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