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|Title: ||Comparison of molecular and antibiotic resistance pro®le methods for the population analysis of Bradyrhizobium spp. (TGx) isolates that nodulate the new TGx soybean cultivars in Africa|
|Authors: ||Abaidoo, Robert C.|
Keyser, H. H.
Singleton, P. W.
|Issue Date: ||2002|
|Publisher: ||Journal of Applied Microbiology|
|Citation: ||Journal of Applied Microbiology 2002, 92, 109-117|
|Abstract: ||Aims: Comparison of molecular and antibiotic resistance pro®le methods to identify an easy
method that can differentiate between strains of introduced Bradyrhizobium japonicum and the
indigenous Bradyrhizobium spp. (TGx) isolates which nodulate the newly developed TGx
soybean cultivars in Africa.
Methods and Results: Restriction fragment length polymorphism (RFLP) of 16S rDNA
generated by ®ve restriction enzymes, banding patterns in Southern hybridization using nod
and nif genes as probes, and resistance patterns of the isolates to nine antibiotics, were used to
group 26 Bradyrhizobium spp. (TGx) isolates and four other Bradyrhizobium strains. The
clusters of isolates obtained from the four grouping methods were all different, although all
methods revealed large genetic diversity among the isolates.
Conclusions: Results indicate that the antibiotic resistance pro®le method is as good as the
three molecular methods used in this study for phylogenetic grouping of the Bradyrhizobium
spp. (TGx) isolates, which may serve as a basis for further characterization of selected isolates
from each group.
Signi®cance and Impact of the Study: The antibiotic resistance pro®le method can be used
as a simple means of assessing genetic variability and grouping of a large number of
Bradyrhizobium spp. (TGx) isolates. Representative isolates from each group can then be
selected for further characterization.|
|Description: ||An article published by Journal of Applied Microbiology 2002, 92, 109-117|
|Appears in Collections:||College of Health Sciences|
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