Mycobacterium ulcerans DNA Not Detected in Faecal Samples from Buruli Ulcer Patients: Results of a Pilot Study

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dc.contributor.authorPhillips, Richard Odame
dc.contributor.authorSarfo, Fred Stephen
dc.contributor.authorLavender, Caroline J.
dc.contributor.authorFyfe, Janet A. M.
dc.contributor.authorJohnson, Paul D. R.
dc.contributor.authoret. al
dc.date.accessioned2020-01-13T11:30:35Z
dc.date.accessioned2023-04-19T01:46:35Z
dc.date.available2020-01-13T11:30:35Z
dc.date.available2023-04-19T01:46:35Z
dc.date.issued2011-05-04
dc.descriptionAn article published byen_US
dc.description.abstractIt has recently been shown that in a Buruli ulcer (BU) endemic region of southeastern Australia, significant numbers of possums (native tree-dwelling marsupials) have clinical BU disease. Furthermore, based on quantitative PCR (qPCR) analysis, animals with BU lesions (and some without) shed M. ulcerans DNA in their faeces, indicative of bacterial loads of up to 108 organisms/gram. These findings led us to propose that humans might also harbour M. ulcerans in their gastrointestinal tract and shed the bacterium in their faeces. We conducted a pilot study and collected faecal swabs from 26 patients with confirmed BU and 31 healthy household controls. Faecal samples were also collected from 10 healthy controls from nonendemic regions in Ghana. All 67 specimens were negative when tested by IS2404 PCR. The detection sensitivity of this method was $104 bacteria per gram (wet-weight) of human faecal material. We conclude that the human gastrointestinal tract is unlikely to be a significant reservoir of M. ulcerans.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationPLoS ONE 6(5): e19611. doi:10.1371/journal.pone.0019611en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/11916
dc.language.isoenen_US
dc.publisherPLoS ONEen_US
dc.titleMycobacterium ulcerans DNA Not Detected in Faecal Samples from Buruli Ulcer Patients: Results of a Pilot Studyen_US
dc.typeArticleen_US
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