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|Title: ||Mycobacterium ulcerans DNA Not Detected in Faecal Samples from Buruli Ulcer Patients: Results of a Pilot Study|
|Authors: ||Phillips, Richard O.|
Sarfo, Fred S.
Lavender, Caroline J.
Fyfe, Janet A. M.
Johnson, Paul D. R.
|Issue Date: ||4-May-2011|
|Publisher: ||PLoS ONE|
|Citation: ||PLoS ONE 6(5): e19611. doi:10.1371/journal.pone.0019611|
|Abstract: ||It has recently been shown that in a Buruli ulcer (BU) endemic region of southeastern Australia, significant numbers of
possums (native tree-dwelling marsupials) have clinical BU disease. Furthermore, based on quantitative PCR (qPCR) analysis,
animals with BU lesions (and some without) shed M. ulcerans DNA in their faeces, indicative of bacterial loads of up to 108
organisms/gram. These findings led us to propose that humans might also harbour M. ulcerans in their gastrointestinal tract
and shed the bacterium in their faeces. We conducted a pilot study and collected faecal swabs from 26 patients with
confirmed BU and 31 healthy household controls. Faecal samples were also collected from 10 healthy controls from nonendemic
regions in Ghana. All 67 specimens were negative when tested by IS2404 PCR. The detection sensitivity of this
method was $104 bacteria per gram (wet-weight) of human faecal material. We conclude that the human gastrointestinal
tract is unlikely to be a significant reservoir of M. ulcerans.|
|Description: ||An article published by|
|Appears in Collections:||College of Health Sciences|
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