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Title: | Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63 |
Authors: | El-Duah, Philip Meyer, Benjamin Oppong, Samuel Kingsley Lamptey, Jones et...al |
Keywords: | Coronavirus ELISA livestock intermediate host immunofluorescence |
Issue Date: | Sep-2018 |
Publisher: | Viruses |
Citation: | Viruses |
Abstract: | Known human coronaviruses are believed to have originated in animals and made use
of intermediate hosts for transmission to humans. The intermediate hosts of most of the human
coronaviruses are known, but not for HCoV-NL63. This study aims to assess the possible role
of some major domestic livestock species as intermediate hosts of HCoV-NL63. We developed
a testing algorithm for high throughput screening of livestock sera with ELISA and confirmation
with recombinant immunofluorescence assay testing for antibodies against HCoV-NL63 in livestock.
Optimization of the ELISA showed a capability of the assay to significantly distinguish HCoV-NL63 from
HCoV-229E (U = 27.50, p < 0.001) and HCoV-OC43 (U = 55.50, p < 0.001) in coronavirus-characterized
sera. Evaluation of the assay with collected human samples showed no significant difference in mean
optical density values of immunofluorescence-classified HCoV-NL63-positive and HCoV-NL63-negative
samples (F (1, 215) = 0.437, p = 0.509). All the top 5% (n = 8) most reactive human samples tested by
ELISA were HCoV-NL63 positive by immunofluorescence testing. In comparison, only a proportion
(84%, n = 42) of the top 25% were positive by immunofluorescence testing, indicating an increased
probability of the highly ELISA reactive samples testing positive by the immunofluorescence assay.
None of the top 5% most ELISA reactive livestock samples were positive for HCoV-NL63-related viruses
by immunofluorescence confirmation. Ghanaian domestic livestock are not likely intermediate hosts of
HCoV-NL63-related coronaviruses. |
Description: | This article has been published in Viruses and is also available at DOI:10.3390/v11010043 |
URI: | http://hdl.handle.net/123456789/15218 |
Appears in Collections: | College of Agric and Natural Resources
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