Browsing by Author "Baah, Kennedy Ameyaw"

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    “Hydroethanol extract and triterpenoids of Senegalia ataxacantha show antiplasmodial activity and the compounds are predicted to inhibit parasite lactate dehydrogenase (pfLDH) as indicated by molecular docking studies”
    (Elsevier, 2024-10) Baah, Kennedy Ameyaw; Acheampong, Akwasi; Amponsah, Isaac Kingsley; Adjei, Silas; Jibira, Yakubu; Nketia, Nketia; Sarpong, Linda Mensah; Kontoh, Emmanuel Quaye; 0000-0003-2481-1126
    Malaria claims over 600,000 deaths annually with a disproportionately high burden in the WHO African Region. The development of parasite resistance has warranted the search for novel anti plasmodial drug candidates. This research aimed at validating the efficacy of Senegalia atax acantha and tracking down its bioactive constituents. In vivo antiplasmodial activity of the extract was assessed using suppressive and curative protocols. Yeast-induced pyrexia was employed to evaluate the antipyretic activity of the extract. In vitro anti-plasmodial activity of isolated compounds was done using SYBR green I fluorescence assay on chloroquine sensitive (3D7) and resistant (Dd2) strains of P falciparum. In silico pharmacokinetic and interactions with parasites lactate dehydrogenase predictions of isolated compounds was conducted through molecular docking studies. Ethanol (70 %) extract of the plant showed in vitro and in vivo anti-plasmodial effect. The extract demonstrated significant (p < 0.05) dose-dependent suppression of 63.39 % and 63.32 % respectively at the highest dose (300 mg kg-1). Artesunate (4 mg kg-1/day) had considerably better curative potential (85.25%). The compounds showed in vitro anti-plasmodial activity in the order lupeol> friedelin/ friedelinol mixture>friedelin>β-sitosterol based on IC50 measurement. Friedelinol and lupeol exhibited higher binding affinities with pfLDH compared to Chloroquine. The extract and acetaminophen (positve control) showed significant (p < 0.05) reduction in rectal temperature compared to the control group. In silico studies of the compounds revealed moderate interactions with some cytochrome P450 metabolizing enzymes.
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    In vitro anthelminthic and anti-inflammatory activities, and GC-MS analysis of methanol and acetone extracts of Mareya micrantha leaves
    (Journal of Pharmacognosy and Phytochemistry, 2024-03) Acheampong, Akwasi; Quartey, Emmanuel; Acquaye, Maud Adoley; Naazo, Abdulai A; Baah, Kennedy Ameyaw; Amankwaa, Lydia Tima; Buah, Patrick; Frimpong, Silas Ofori; 0000-0003-2481-1126
    Mareya micrantha, a medicinal plant, is used to treat pains, wounds, worm infestations and gastrointestinal disorders. The aim of this research was to investigate the anthelmintic and anti-inflammatory properties of the methanol and acetone extracts of Mareya micrantha leaves. Phytochemical screening was performed using standard methods with GC-MS used for the identification of the phytochemicals. Egg albumen denaturation was used for the determination of the anti-inflammatory (in vitro) activities of the extracts. Anthelmintic activity (in vitro) of the extracts was investigated against Millsonia ghanensis. Phytochemical investigation revealed the presence of phenols, terpenoids, polyphenols, flavonoids, tannins, steroids, saponins, and glycosides. Twenty phytochemicals, most of which have known bioactivities, were identified for each extract with five being common to them, and they are n-hexadecanoic acid, Tributyl acetyl citrate, hexadecanoic acid methyl ester, 3, 7, 11, 15-tetramethyl-2-hexadecen-1-ol, and 2, 2, 4-trimethyl-3-(3, 8, 12, 16-tetramethyl-heptadeca-3, 7, 11, 15-tetraethyl)-cyclohexanol. The extracts had anti-inflammatory activity. The anthelmintic activity of the extracts was significantly higher than mebendazole-treated helminths. The outcome of this study points to the fact that Mareya micrantha could be exploited as a source of potential drug candidates against helminthic and microbial infection as well as inflammation and oxidative stress.