Browsing by Author "Kobina, Paintsil Ellis"

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    Susceptibility pattern of some bacterial isolates from chronic Wounds to selected local brands of antibiotics
    (KNUST, 2019-06) Kobina, Paintsil Ellis
    Chronic wounds continue to be a challenging problem causing morbidity and mortality to mankind and represent a considerable healthcare burden globally. Despite the fact that antibiotics are not the first choice of treatment for chronic wounds, they remain widely used and abused. Also, because of the high demand of antibiotics, substandard and counterfeit antibiotics are getting into the global supply chain and fuelling resistance. Antibiotic resistance impact negatively on chronic wounds by increasing the hospital cost, economic cost and the hospitalization period needed for treatment. The aim of this study was to identify the susceptibility pattern of some bacteria isolates from chronic wounds to selected local brands of antibiotics commonly used in the treatment of these wounds. A total of 260 wound swab samples were collected from chronic wound patients attending SPH and NGH and inoculated into appropriate media. Bacteria pathogens were isolated, identified and characterized using standard microbiological techniques. Seven local brands of commonly used antibiotics identified were bought and tested against Staphylococcus aureus and Pseudomonas spp. Antimicrobial susceptibility testing of the clinical isolates was done according to CLSI guidelines. Identification of MRSA and ESBL was also done. A total of 211 (81.2%) wound swab samples produced bacterial growth and 237 isolates were recovered. Staphylococcus aureus (30.8%) was the predominate organism isolated, followed by Pseudomonas spp. (24.9%). Majority of the patients (87.31%) with chronic wounds received antibiotics, metronidazole and cefuroxime are the most common (21.74%) antibiotics prescribed. 30.47% and 25.76% of the patients investigated from NGH and SPH respectively had used herbs on their wounds. The results identified 21 (28.8%) out of 73 Staphylococcus aureus isolates as MRSA and 35% of the Pseudomonas spp. to be ESBL. Five out of the seven local brands of antibiotics tested showed biological activity similar to the standard commercial controls. In this study, the observed susceptibility percentages and mean zones of inhibition of most local brands of antibiotics tested were statistically comparable (p> 0.05) to the standard controls used.