Browsing by Author "Osei Akoto, Clement"

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    Isolation and Identification of Flavanone Derivative Eriodictyol from the Methanol Extract of Afzelia africana Bark and Its Antimicrobial and Antioxidant Activities
    (Evidence-Based Complementary and Alternative Medicine, 2023) Vigbedor, Bright Yaw; Osei Akoto, Clement; Neglo, David; 0000-0003-3955-9883; 0000-0003-2717-2753; 0000-0003-2547-5021
    Background. Afzelia africana is a plant species with well-documented ethnobotanical and medicinal properties. The plant is reported to have various secondary metabolites and had been applied for the treatment of various diseased conditions. Objectives. The study objectives include fractionation, isolation, purifcation, and characterization of eriodictyol from the bark of A. africana, and the determination of its antimicrobial and antioxidant activities. Methodology. Te series of methodologies that were employed include fractionations and purifcation (column chromatography), characterization (HPLC, LC-MS, IR, 1 H, 13C, DEPT-135, HSQC, and HMBC), antimicrobial assays (microbroth dilution and checkerboard assay), and antioxidant activities assays (ABTS and DPPH scavenging capacity). Results. The study reports the identifcation and characterization of eriodictyol from the bark of A. africana which exhibited potent antioxidant activities against ABTS and DPPH radicals with scavenging capacities (SC50) of 2.14 ± 0.05 and 2.51 ± 0.06 µg/mL, respectively. The compound exhibited its antimicrobial activity by reporting good bacteriostatic activities (MBC/MIC> 4) against Staphylococcus aureus (SA), methicillin-resistant Staphylococcus aureus (MRSA), and fuconazole-resistant Candida albicans (CA2). Moreover, a broad spectrum of bactericidal efects (MBC/MIC ≤ 4) was reported against Streptococcus mutans (SM), Escherichia coli (EC), Bacillus subtilis (BS), Klebsiella pneumonia (KP), Pseudomonas aeruginosa (PA), Salmonella typhi (ST), and standard Candida albicans (CA1). The compound further exhibited synergistic efects against EC, KP, ST, and MRSA; ST; and CA2 when combined with ciprofoxacin, tetracycline, and nystatin, respectively. However, antagonistic efects were observed against PA and CA1 when combined with ciprofoxacin and ketoconazole, respectively. Conclusion. Te study reports for the frst time the identifcation of eriodictyol from the bark of A. africana which exhibited signifcant antioxidant and antimicrobial properties.