Propagation of Bacillus thuringiensis israelensis on cowpea vigna unguiculata.

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The possibility of using Cowpea seeds as protein base for the cultivation of Bacillus thuring israelensis is investigated. Cowpeas contain carbohydrates, proteins and mineral, nutrients needed for the growth and sporulation of the bacillus. Baciillus thuringiensis isaelensis on Nutrient broth and Cowpeas formulations supplemented with various concentrations of monosodium glutamate. Monosodium glutamate at a concentration of 0.4% gave the beet growth of the organism on Cowpea medium whilst a 5% concentration gave the poorest growth. Dilutions of the Bacillus in Cowpea medium and in Nutrient broth ranging between 10-7 and 10-7 caused deaths in fourth instar larvae of Aedes aegypti. The L.C50 for the organism fermented in Monosodium glutamate in Cowpea medium was 10-4.5 whilst that of Nutrient broth was 10-4.0. Monosodium glutamate in Cowpea medium warn found to be a more efficacious and less costly medium for the propagation of the Bacillus as a microbial insecticide. Monosodium glutamate in Cowpea medium formulated as 0.4% Monosodium glutamate in 99.6% Cowpea extract barn bean recommended as an alternative medium to the rather costly nutrient broth as a protein base for the propagation of Bacillus thuringiensis israelensis as a microbial insecticide.
A thesis submitted to the Board of Postgraduate Studies, Kwame Nkrumah University of Science and Technology, Kumasi, in partial fulfilment of the requirements for the award of the Degree of Master of Philosophy in Biological Sciences, 1986