Distribution of species of the anopheles gambiae complex and their pyrethroid insecticide knock down resistance status in the Kumasi metropolis.

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The Anopheles species play a central role in malaria transmission. Several methods are available to map out the distribution and distinction of various species of the Anopheles in the tropics and sub-tropical regions of the world. However the advent of molecular technology, polymerase chain reaction assay, which is faster and based on genotypic diversity of the species, simplified this research. This study was set out to identify and determine the distribution of Anopheles gambiae s.s, (the most prevalent malaria vector) within the selected communities in Kumasi. DNA was extracted from Anopheles mosquitoes larvae and used as template in polymerase chain reaction (PCR), for each specimen. Knock down resistance (Kdr) was further carried out on the samples. Seventy six out of 100 larvae collected were identified as gambiae s.s. Twenty six out of 50 An. gambiae samples were found to posses Kdr gene with remaining 24 samples being susceptible.
A thesis submitted to the Department of Biochemistry and Biotechnology Kwame Nkrumah University of Science and Technology in partial fulfillment for the degree of Master of Science in Biotechnology, 2008